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Giovanna Roncador

Name of the laboratory

Monoclonal Antibodies Unit

Centro Nacional de Investigaciones Oncológicas

Calle Melchor Fernández Almagro 3

28029 Madrid, Spain

34 912 246 900

Members of the laboratory

Unit Leader

Giovanna Roncador groncador@cnio.es

Technicians

Lorena Maestre lmaestre@cnio.es

Mar López Colomer mmlopez@cnio.es

Ana Isabel  Reyes García aireyes@cnio.es

Sherezade Jiménez

Laboratory activity

Hybridoma technology for the production of monoclonal antibodies represents one of the most relevant methodological advances in biomedicine. The availability of monoclonal antibodies has significantly improved our knowledge about cell biology, opening new possibilities for basic and applied research. Its use in medicine has also improved the diagnostics, prevention and treatment of a large number of diseases.

The Monoclonal Antibodies (mAb) Unit provides CNIO Research Groups with the “à la carte”, generation of mAbs which can then be used as tools to characterise new pathways involved in cancer development. We are highly specialised in mouse and rat monoclonal antibodies production. The Unit also offers mAbs production in gene-inactivated mice, mAb characterisation and validation, medium-scale mAb production and a service of Mycoplasma testing for the cell culture facility.

Research activities

Mouse specific antibodies: During the latest years, genetic approaches have been extensively used at the CNIO for the functional analysis of genes directly implicated in the process of tumor transformation and progression. The study of mouse models has been hampered by the absence of reliable mouse-specific antibodies to be used on paraffin-embedded tissues. For this reason, and in collaboration with the CNIO Comparative Pathology Unit, we have tested and developed a panel of high quality mouse specific mAbs, able to detect their target molecules on paraffin mouse tissue sections by immunohistochemistry.

Rat mAb: Many protein targets relevant for signal transduction and disease are highly conserved between mice and humans, and can therefore be recognized as self-antigens by a mouse host. To overcome this problem, this year we have made available in our unit a new service of production of mAbs in rats. This will provide CNIO investigators with a powerful tool to study cell biology and cancer related processes in mouse models. The mAbs against mouse protein and produced in rat to date are directed against the following proteins: Beta-Gal, p15,p16,p21,p53, Hes-1and Nanog.

Techniques available

• monoclonal antibody production in mice
• polyclonal antibody production
• cloning technique
• antibody characterization
• development of double and triple immunostaining techniques
• mycoplasma testing
• genetic immunization

Publications

• Ramos-Medina R, Montes-Moreno S, Maestre L, Cañamero M, Rodríguez-Pinilla M, Martínez-Torrecuadrada J, Piris MA, Majid A, Dyer MJ, Pulford K, Roncador G (2013). BCL7A protein expression in normal and malignant lymphoid tissues
  Br J Haemato  160(1):106-9.

• Montes-Moreno S, Ramos-Medina R, Martínez-López A, Barrionuevo Cornejo C, Parra Cubillos A, Quintana-Truyenque S, Rodriguez Pinilla SM, Pajares R, Sanchez-Verde L, Roncador G, Piris MA (2013). SPIB, a novel immunohistochemical marker for human blastic plasmacytoid dendritic cell neoplasms: characterization of its expression in major haematolymphoid neoplasms.
  Blood 24;121(4):643-7

• Burnichon N, Cascón A, Schiavi F, Morales NP, Comino-Méndez I, Abermil N, Inglada-Pérez L, de Cubas AA, Amar L, Barontini M, de Quirós SB, Bertherat J, Bignon YJ, Blok MJ, Bobisse S, Borrego S, Castellano M, Chanson P, Chiara MD, Corssmit EP, Giacchè M, de Krijger RR, Ercolino T, Girerd X, Gómez-García EB, Gómez-Graña A, Guilhem I, Hes FJ, Honrado E, Korpershoek E, Lenders JW, Letón R, Mensenkamp AR, Merlo A, Mori L, Murat A, Pierre P, Plouin PF, Prodanov T, Quesada-Charneco M, Qin N, Rapizzi E, Raymond V, Reisch N, Roncador G, Ruiz-Ferrer M, Schillo F, Stegmann AP, Suarez C, Taschin E, Timmers HJ, Tops CM, Urioste M, Beuschlein F, Pacak K, Mannelli M, Dahia PL, Opocher G, Eisenhofer G, Gimenez-Roqueplo AP, Robledo M (2012). MAX mutations cause hereditary and sporadic pheochromocytoma and paraganglioma.
  Clin Cancer Res 15, 2828-37.

• Patel S, Chen H, Monti L, Gould E, Haralambieva E, Schmid J, Toomey D, Woessmann W, Roncador G, Hatton CS, Liggins AP, Taramelli R, Banham AH, Acquati F, Murphy D, Pulford K (2012). RNASET2 an autoantigen in anaplastic large cell lymphoma identified by protein array analysis.
   J Proteomics 18, 5279-92

• Kempf W, Kazakov DV, Cipolat C, Kutzner H, Roncador G, Tomasini D (2012). CD4/CD8 Double Negative Mycosis Fungoides With PD-1 (CD279) Expression-A Disease of Follicular Helper T-Cells?
  Am J dermatopathol 7, 757-761

• Martin-Arruti M, Vaquero M, Díaz de Otazu R, Zabalza I, Ballesteros J, Roncador G, García-Orad A (2012). Bcl-2 and BLIMP-1 expression predict worse prognosis in gastric diffuse large B cell lymphoma (DLCBL) while other markers for nodal DLBCL are not useful.
  Histopathology 5, 785-92

• Guijarro MV, Vergel M, Marin JJ, Muñoz-Galván S, Ferrer I, Cajal SR, Roncador G, Blanco-Aparicio C, Carnero A (2012). p38α limits the contribution of MAP17 to cancer progression in breast tumors.
  Oncogene 11,4447-59.

• Comino-Méndez I, Gracia-Aznárez FJ, Schiavi F, Landa I, Leandro-García LJ, Letón R, Honrado E, Ramos-Medina R, Caronia D, Pita G, Gómez-Graña A, de Cubas AA, Inglada-Pérez L, Maliszewska A, Taschin E, Bobisse S, Pica G, Loli P, Hernández-Lavado R, Díaz JA, Gómez-Morales M, González-Neira A, Roncador G, Rodríguez-Antona C, Benítez J, Mannelli M, Opocher G, Robledo M, Cascón A (2011). Exome sequencing identifies MAX mutations as a cause of hereditary pheochromocytoma
  Nat Genet  Jun 19;43(7):663-7.

• Yamamoto Y, Negoro T, Hoshi A, Wakagi A, Shimizu S, Banham AH, Ishii M, Akiyama H, Kiuchi Y, Sunaga S, Tobe T, Roncador G, Itabashi K, Nakano Y (2011).Impaired Ca Regulation of CD4(+)CD25(+) Regulatory T Cells from Pediatric Asthma.
  Int Arch Allergy Immunol. May 17;156(2):148-158.

• Ramos-Medina R, Montes-Moreno S, Maestre L, Cañamero M, Rodriguez-Pinilla M, Lázaro A, Roncador G. Immunohistochemical analysis of HLDA9 Workshop antibodies against cell-surface molecules in reactive and neoplastic lymphoid tissues.
  Immunol Lett. 2011 Jan 30;134(2):150-6

• Churchill HR, Roncador G, Warnke RA, Natkunam Y (2010). Programmed death 1 expression in variant immunoarchitectural patterns of nodular lymphocyte predominant Hodgkin lymphoma: comparison with CD57 and lymphomas in the differential diagnosis.
  Hum Pathol Sep 6.

• Ballabio E, Chi J, Roncador G, Banham AH, Hatton CS, Lawrie CH (2010). Comparison of Choi and Hans' algorithms by immunohistochemistry and quantitative reverse transcriptase-PCR - letter.
  Clin Cancer Res  Jul 15; 16(14):3805-6.

• Montes-Moreno S, Gonzalez-Medina AR, Rodriguez-Pinilla SM, Maestre L, Sanchez-Verde L, Roncador G, Mollejo M, García JF, Menarguez J, Montalbán C, Ruiz-Marcellan MC, Conde E, Piris MA (2010). Aggressive large B-cell lymphoma with plasma cell differentiation: immunohistochemical characterization of plasmablastic lymphoma and diffuse large B-cell lymphoma with partial plasmablastic phenotype.
  Haematologica Aug; 95(8):1342-9.

• Martín-Pérez D, Sánchez E, Maestre L, Suela J, Vargiu P, Di Lisio L, Martínez N, Alves J, Piris MA, Sánchez-Beato M (2010). Deregulated expression of the polycomb-group protein SUZ12 target genes characterizes mantle cell lymphoma.
  Am J Pathol Aug; 177(2):930-42.

• Campbell AJ, Lyne L, Brown PJ, Launchbury RJ, Bignone P, Chi J, Roncador G, Lawrie CH, Gatter KC, Kusec R, Banham AH (2010). Aberrant expression of the neuronal transcription factor FOXP2 in neoplastic plasma cells.
  J Haematol Apr; 149(2):221-30.

• Herreros B, Rodríguez-Pinilla SM, Pajares R, Martínez-Gónzalez MA, Ramos R, Munoz I, Montes-Moreno S, Lozano M, Sánchez-Verde L, Roncador G, Sánchez-Beato M, de Otazu RD, Pérez-Guillermo M, Mestre MJ, Bellas C, Piris MA (2010). Proliferation centers in chronic lymphocytic leukemia: the niche where NF-kappaB activation takes place.
  Leukemia Apr; 24(4):872-6.

• Wahlin BE, Aggarwal M, Montes-Moreno S, Gonzalez LF, Roncador G, Sanchez-Verde L, Christensson B, Sander B, Kimby E (2010). A unifying microenvironment model in follicular lymphoma: outcome is predicted by programmed death-1--positive, regulatory, cytotoxic, and helper T cells and macrophages.
  Clin Cancer Res Jan 15; 16(2):637-50.

• Simpson N, Gatenby PA, Wilson A, Malik S, Fulcher DA, Tangye SG, Manku H, Vyse TJ, Roncador G, Huttley GA, Goodnow CC, Vinuesa CG, Cook MC (2010). Expansion of circulating T cells resembling follicular helper T cells is a fixed phenotype that identifies a subset of severe systemic lupus erythematosus.
  Arthritis RheumJan; 62(1):234-44.

• Acebo P, Giner D, Calvo P, Blanco-Rivero A, Ortega AD, Fernández PL, Roncador G, Fernández-Malavé E, Chamorro M, Cuezva JM (2009).  Cancer abolishes the tissue type-specific differences in the phenotype of energetic metabolism.
  Transl Oncol 18;2(3):138-45

• Kanellis G, Roncador G, Arribas A, Mollejo M, Montes-Moreno S, Maestre L, Campos-Martin Y, Ríos Gonzalez JL, Martinez-Torrecuadrada JL, Sanchez-Verde L, Pajares R, Cigudosa JC, Martin MC, Piris MA (2009). Identification of MNDA as a new marker for nodal marginal zone lymphoma.
  Leukemia 23(10):1847-57.

• Ortiz-Muchotrigo N, Rodríguez-Pinilla SM, Ramos R, Montes-Moreno S, González-López MA, Armesto-Pérez A, Roncador G, Piris MA (2009). Follicular T-cell lymphoma: description of a case with characteristic findings suggesting it is a different condition from AITL.
  Histopathology 54(7):902-4.

• Rodriguez-Justo M, Attygalle AD, Munson P, Roncador G, Marafioti T, Piris MA (2009). Angioimmunoblastic T-cell lymphoma with hyperplastic germinal centres: a neoplasia with origin in the outer zone of the germinal centre? Clinicopathological and immunohistochemical study of 10 cases with follicular T-cell markers.
  Mod Pathol 22(6):753-61

• Huang Y, Moreau A, Dupuis J, Streubel B, Petit B, Le Gouill S, Martin-Garcia N, Copie-Bergman C, Gaillard F, Qubaja M, Fabiani B, Roncador G, Haioun C, Delfau-Larue MH, Marafioti T, Chott A, Gaulard P (2009). Peripheral T-cell lymphomas with a follicular growth pattern are derived from follicular helper T cells (TFH) and may show overlapping features with angioimmunoblastic T-cell lymphom.
  Am J Surg Pathol 33(5):682-90

• Carreras J, Lopez-Guillermo A, Roncador G, Villamor N, Colomo L, Martinez A, Hamoudi R, Howat WJ, Montserrat E, Campo E (2009). High numbers of tumor-infiltrating programmed cell death 1-positive regulatory lymphocytes are associated with improved overall survival in follicular lymphoma.
  J Clin Oncol 20;27(9):1470-6.

• Maestre L, Tooze R, Cañamero M, Montes-Moreno S, Ramos R, Doody G, Boll May, Barrans S, Baena S, Piris MA, and  Roncador G (2009). Expression pattern of XBP-1s in Human B cell Lymphomas.
  Haematologica 94(3):419-22.

• Rodríguez-Pinilla SM, Atienza L, Murillo C, Pérez-Rodríguez A, Montes-Moreno S, Roncador G, Pérez-Seoane C, Domínguez P, Camacho FI, Piris MA. (2008). Peripheral T-cell Lymphoma With Follicular T-cell Markers.
  Am J Surg Pathol. Sep 5.

• Nam-Cha SH, Roncador G, Sanchez-Verde L, Montes-Moreno S, Acevedo A, Domínguez-Franjo P, Piris MA. (2008). PD-1, a follicular T-cell marker useful for recognizing nodular lymphocyte-predominant Hodgkin lymphoma.
  Am J Surg Pathol. Aug;32(8):1252-7.

• Montes-Moreno S, Roncador G, Maestre L, Martínez N, Sanchez-Verde L, Camacho FI, Cannata J, Martinez-Torrecuadrada JL, Shen Y, Chan WC, Piris MA. (2008). Gcet1 (centerin), a highly restricted marker for a subset of germinal center-derived lymphomas.
  Blood. Jan 1;111(1):351-8.