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mAbs Against MOUSE Protein

p27

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:rat anti mouse
CLONE NAME:SON82B
PROTEIN:Mus musculus cyclin-dependent kinase inhibitor 1B (Cdkn1b)
ANTIGEN USED:HIS-mp27 recombinant protein
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

Cyclin-dependent kinase inhibitor 1B (p27Kip1) is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1.

REFERENCES

Muñoz-Espín D, Cañamero M, Maraver A, Gómez-López G, Contreras J, Murillo-Cuesta S, Rodríguez-Baeza A, Varela-Nieto I, Ruberte J, Collado M, Serrano M. Programmed cell senescence during mammalian embryonic development. Cell. 2013 Nov 21;155(5):1104-18.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
SON82B 1:100 supernatant / nuclear
Immunofluorescence
Enlarge image
SON82B mAb in transfected cells
Nuclear and cytoplasmic staining of SON82B mAb in transfected HEK293-V5-mp27 cells. HEK293-V5-mp57 was used as negative control and anti-V5 was used as positive control.
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SON82B mAb in mouse paraffin tissues.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
SON82B neat supernatant supernatant / 27kDa 27kDa
Immunoprecipitation
Enlarge image
Western Blotting of mp27 (SON82B) in transfected cells
Lane 1 Hek-mp27 (30цg) (+)
Lane 2 Hek-mp16 (30цg) (-)

Anti-tubulin was used as loading control