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mAbs Against HUMAN Protein

BCL2

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:bcl-2/100
PROTEIN:B-cell lymphoma 2
PROTEIN WEB:http://omim.org/entry/151430
ANTIGEN USED:Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC WB

DESCRIPTION

The pro-survival protein BCL2 derives its name from B-cell lymphoma 2, as it is the second member of a range of proteins initially described in chromosomal translocations involving chromosomes 14 and 18 in follicular lymphomas. Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation.
Pezzella F, Tse AG, Cordell JL, Pulford KA, Gatter KC, Mason DY.
Am J Pathol. 1990 Aug;137(2):225-32.
PMID: 2201196
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1877598/

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
bcl-2/100 neat supernatant Tris EDTA DAKO Envision Tonsil /
Immunofluorescence
Enlarge image
bcl-2/100 Tonsil
Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
bcl-2/100 Tonsil
Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 20x power image