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mAbs Against HUMAN Protein

CD3

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:PC3/188A
PROTEIN:CD3
PROTEIN WEB:https://www.omim.org/entry/186830
ANTIGEN USED:Peptide sequence (156-168) from the intracytoplasmic portion of the epsilon-chain of CD3
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Bovine, Chicken, Horse, Guinea Pig, Opossum, Pig, Primate, Rat and Rabbit
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC WB

DESCRIPTION

The CD3 complex, composed of five CD3 polypeptide chains, associates with the T cell antigen receptor (TCR) and is found on all mature human T lymphocytes. CD3 can be used for the identification of normal and malignant T lymphocytes in paraffin embedded or frozen tissue sections. PC3/188A also detects CD3 in mammalian tissues.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

J Immunol. 1993 Jun 15;150(12):5429-35.
Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
Jones M1, Cordell JL, Beyers AD, Tse AG, Mason DY.
PMID: 8515069
https://www.ncbi.nlm.nih.gov/pubmed/?term=PMID%3A+8515069

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
PC3 188A neat supernatant Tris EDTA dako Envision Tonsil /
Immunofluorescence
Enlarge image
Pc3/188A Tonsil
Tonsil stained with neat supernatant from PC3/188A hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
PC3/188A Tonsil
Tonsil stained with neat supernatant from PC3/188A hybridoma, detection using Dako Envision kit, 20x power image