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mAbs Against HUMAN Protein

CD45RA

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:4KB5
PROTEIN:Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RA)
PROTEIN WEB:https://www.omim.org/entry/151460
ANTIGEN USED:B cell lymphoma
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1 kappa
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IP WB

DESCRIPTION

PTPRC (CD45) is a transmembrane tyrosine phosphatase that is present on all leukocytes. PTPRC regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). CD45RA is an isoform of the PTPRC (CD45) protein which is selectively expressed on B cells.

REFERENCES

Pulford KAF et al. 1987. In: McMichael A, et al (eds) Leucocyte Typing III. Oxford University Press, Oxford, New York and Tokyo, p 828

Blood. 2005 Apr 15;105(8):3295-302. Epub 2004 Dec 30.
A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma.
Li FJ1, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM.
PMID: 15626731
https://www.ncbi.nlm.nih.gov/pubmed/15626731

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
4KB5 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
4KB5 Tonsil
Tonsil stained with neat supernatant from 4KB5 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
4KB5 Tonsil
Tonsil stained with neat supernatant from 4KB5 hybridoma, detection using Dako Envision kit, 20x power image