mAbs Against HUMAN Protein
CD45RA
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | 4KB5 |
| PROTEIN: | Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RA) |
| PROTEIN WEB: | https://www.omim.org/entry/151460 |
| ANTIGEN USED: | B cell lymphoma |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 kappa |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC IP WB |
Description
PTPRC (CD45) is a transmembrane tyrosine phosphatase that is present on all leukocytes. PTPRC regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). CD45RA is an isoform of the PTPRC (CD45) protein which is selectively expressed on B cells.
References
Pulford KAF et al. 1987. In: McMichael A, et al (eds) Leucocyte Typing III. Oxford University Press, Oxford, New York and Tokyo, p 828
Blood. 2005 Apr 15;105(8):3295-302. Epub 2004 Dec 30.
A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma.
Li FJ1, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM.
PMID: 15626731
https://www.ncbi.nlm.nih.gov/pubmed/15626731
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| 4KB5 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- 4KB5 Tonsil
- Tonsil stained with neat supernatant from 4KB5 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- 4KB5 Tonsil
- Tonsil stained with neat supernatant from 4KB5 hybridoma, detection using Dako Envision kit, 20x power image