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mAbs Against HUMAN Protein

CD68

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:KP1
PROTEIN:CD68
PROTEIN WEB:https://www.omim.org/entry/153634
ANTIGEN USED:Lysosomal contents of lung macrophages
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IF IP WB

DESCRIPTION

CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. KP1 is widely used to detect macrophages in paraffin embedded tissue sections.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

J Clin Pathol. 1989 Apr;42(4):414-21.
KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.
Pulford KA1, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, Mason DY.
PMID: 2654191
https://www.ncbi.nlm.nih.gov/pubmed/2654191

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
KP1 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
KP1 Tonsil
Tonsil stained with neat supernatant from KP1 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
KP1 Tonsil
Tonsil stained with neat supernatant from KP1 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
KP1 Tonsil
Tonsil stained with neat supernatant from KP1 hybridoma, detection using Dako Envision kit, 20x power image