mAbs Against HUMAN Protein
CD68
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | KP1 |
| PROTEIN: | CD68 |
| PROTEIN WEB: | https://www.omim.org/entry/153634 |
| ANTIGEN USED: | Lysosomal contents of lung macrophages |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC IF IP WB |
Description
CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. KP1 is widely used to detect macrophages in paraffin embedded tissue sections.
Publication describing antibody characterization/validation
J Clin Pathol. 1989 Apr;42(4):414-21.
KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.
Pulford KA1, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, Mason DY.
PMID: 2654191
https://www.ncbi.nlm.nih.gov/pubmed/2654191
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| KP1 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- KP1 Tonsil
- Tonsil stained with neat supernatant from KP1 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- KP1 Tonsil
- Tonsil stained with neat supernatant from KP1 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- KP1 Tonsil
- Tonsil stained with neat supernatant from KP1 hybridoma, detection using Dako Envision kit, 20x power image