mAbs Against HUMAN Protein
CD79a
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | HM57 |
| PROTEIN: | CD79a |
| PROTEIN WEB: | https://www.omim.org/entry/112205 |
| ANTIGEN USED: | Synthetic peptide of 14 amino acids representing residues 202-216 of the human mb-1 cDNA sequence. |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Bovine, Chicken, Horse, Human, Guinea Pig, Mouse, Opossum, Pig, Primate, Rat and Rabbit |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC IP WB |
Description
The B cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, non covalently associated with membrane bound immunoglobulins on B cells. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B cell lymphomas, in B cell lines, the majority of acute leukemias of precursor B cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling. CD79a is widely used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissues sections.
Publication describing antibody characterization/validation
J Immunol. 1991 Dec 1;147(11):2474-82.
The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
Mason DY1, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.
PMID: 1747162
https://www.ncbi.nlm.nih.gov/pubmed/1747162
References
J Immunol. 1991 Jun 1;146(11):3881-8.
The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene.
van Noesel CJ1, van Lier RA, Cordell JL, Tse AG, van Schijndel GM, de Vries EF, Mason DY, Borst J.
PMID: 2033258
https://www.ncbi.nlm.nih.gov/pubmed/2033258
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| HM57 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- HM57 Tonsil
- Tonsil stained with neat supernatant from HM57 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- HM57 Tonsil
- Tonsil stained with neat supernatant from HM57 hybridoma, detection using Dako Envision kit, 10x power image