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mAbs Against HUMAN Protein

CD79a

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:HM57
PROTEIN:CD79a
PROTEIN WEB:https://www.omim.org/entry/112205
ANTIGEN USED:Synthetic peptide of 14 amino acids representing residues 202-216 of the human mb-1 cDNA sequence.
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Bovine, Chicken, Horse, Human, Guinea Pig, Mouse, Opossum, Pig, Primate, Rat and Rabbit
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IP WB

DESCRIPTION

The B cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, non covalently associated with membrane bound immunoglobulins on B cells. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B cell lymphomas, in B cell lines, the majority of acute leukemias of precursor B cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling. CD79a is widely used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissues sections.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

J Immunol. 1991 Dec 1;147(11):2474-82.
The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
Mason DY1, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.
PMID: 1747162
https://www.ncbi.nlm.nih.gov/pubmed/1747162

REFERENCES

J Immunol. 1991 Jun 1;146(11):3881-8.
The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene.
van Noesel CJ1, van Lier RA, Cordell JL, Tse AG, van Schijndel GM, de Vries EF, Mason DY, Borst J.
PMID: 2033258
https://www.ncbi.nlm.nih.gov/pubmed/2033258

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
HM57 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
HM57 Tonsil
Tonsil stained with neat supernatant from HM57 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
HM57 Tonsil
Tonsil stained with neat supernatant from HM57 hybridoma, detection using Dako Envision kit, 10x power image