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mAbs Against HUMAN Protein

CD79a

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:JCB117
PROTEIN:CD79a
PROTEIN WEB:https://www.omim.org/entry/112205
ANTIGEN USED:Synthetic peptide of 14 amino acids representing residues 202-216 of the human mb-1 cDNA sequence
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC WB

DESCRIPTION

The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling. CD79a is widely used as an adjunct to CD20 as a biomarker for normal and neoplastic B-cells in tissues sections.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Blood. 1995 Aug 15;86(4):1453-9.
CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples.
Mason DY1, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H, et al.
PMID: 7632952
https://www.ncbi.nlm.nih.gov/pubmed/7632952

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
JCB117 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
JCB117 Tonsil
Tonsil stained with neat supernatant from JCB117 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
JCB117 Tonsil
Tonsil stained with neat supernatant from JCB117 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
JCB117 Tonsil
Tonsil stained with neat supernatant from JCB117 hybridoma, detection using Dako Envision kit, 20x power image