mAbs Against HUMAN Protein
CD79a
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | JCB117 |
| PROTEIN: | CD79a |
| PROTEIN WEB: | https://www.omim.org/entry/112205 |
| ANTIGEN USED: | Synthetic peptide of 14 amino acids representing residues 202-216 of the human mb-1 cDNA sequence |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | IHC WB |
Description
The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling. CD79a is widely used as an adjunct to CD20 as a biomarker for normal and neoplastic B-cells in tissues sections.
Publication describing antibody characterization/validation
Blood. 1995 Aug 15;86(4):1453-9.
CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples.
Mason DY1, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H, et al.
PMID: 7632952
https://www.ncbi.nlm.nih.gov/pubmed/7632952
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| JCB117 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- JCB117 Tonsil
- Tonsil stained with neat supernatant from JCB117 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- JCB117 Tonsil
- Tonsil stained with neat supernatant from JCB117 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- JCB117 Tonsil
- Tonsil stained with neat supernatant from JCB117 hybridoma, detection using Dako Envision kit, 20x power image