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mAbs Against HUMAN Protein

CD9

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:P1/33/2
PROTEIN:CD9
PROTEIN WEB:https://www.omim.org/entry/143030
ANTIGEN USED:Acute lymphoblastic leukaemia cells
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC

DESCRIPTION

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and neurons and glial cells in the peripheral nervous system. CD9 mediates platelet aggregation and activation via FcgRIIa. It may play a role in cell migration.Antibodies against CD9 have utility in characterisation of acute leukaemias and in some cases AML and for functional studies on platelets

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Jennings et al. 1993. CD9 cluster workshop report: cell surface binding and functional analysis. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 2, Oxford University Press, Oxford, New York and Tokyo, p 1249-51

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
P1/33/2 neat supernatant Citrate Dako Envision Tonsil /
Immunofluorescence
Enlarge image
P1/33/2 Tonsil
Tonsil stained with neat supernatant from P1/33 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
P1/33/2 Tonsil
Tonsil stained with neat supernatant from P1/33/2 hybridoma, detection using Dako Envision kit, 20x power image