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mAbs Against HUMAN Protein

CEACAM5&6

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:By114
PROTEIN:Carcinoembryonic antigen-related cell adhesion molecules 5 & 6
PROTEIN WEB:https://www.omim.org/entry/114890
ANTIGEN USED:B cell lymphoma cells
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1 kappa
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IF IP WB

DESCRIPTION

CEACAM is a member of the carcinoembryonic antigen family (a diagnostic marker for many cancers). Clone By114 reacts with a phosphatidylinositol (PI) linked protein with a molecular mass of 90 kD on granulocyte and squamous epithelium. This antibody is useful in differentiating normal cells from neoplastic cells of granulocyte origin. CD66CE is useful in differentiating normal cells from neoplastic cells of granulocyte origin. By114 may be used to study Paroxysmal Nocturnal Hemoglobinuria (PNH).

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Br J Haematol. 1993 Jan;83(1):30-8.
Antibody By114 is selective for the 90 kD PI-linked component of the CD66 antigen: a new reagent for the study of paroxysmal nocturnal haemoglobinuria.
Mayne KM1, Pulford K, Jones M, Micklem K, Nagel G, van der Schoot CE, Mason DY.
PMID: 8435334
https://www.ncbi.nlm.nih.gov/pubmed/8435334

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
BY114 neat supernatant Citrate Dako Envision Tonsil /
Immunofluorescence
Enlarge image
BY114 Tonsil
Tonsil stained with neat supernatant from BY114 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
BY114 Tonsil
Tonsil stained with neat supernatant from BY114 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
BY114 Tonsil
Tonsil stained with neat supernatant from BY114 hybridoma, detection using Dako Envision kit, 20x power image