mAbs Against HUMAN Protein
CR1
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | To5 |
| PROTEIN: | Complement Receptor Type 1 (CR1, CD35) |
| PROTEIN WEB: | https://www.omim.org/entry/120620 |
| ANTIGEN USED: | C3b receptor-enriched precipitates from tonsil cell membranes |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC IF WB |
Description
CR1 is found on many leucocytes and binds C3b and C4b. To5 may be used in studies of immune complex mediated glomerulopathies.
Publication describing antibody characterization/validation
Immunology. 1982 Apr;45(4):645-53.
Human complement (C3b) receptors defined by a mouse monoclonal antibody.
Gerdes J, Naiem M, Mason DY, Stein H.
PMID: 7068171
https://www.ncbi.nlm.nih.gov/pubmed/7068171
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| To5 | neat | supernatant | Citrate | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- To5 Tonsil
- Tonsil stained with neat supernatant from To5 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- To5 Tonsil
- Tonsil stained with neat supernatant from To5 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- To5 Tonsil
- Tonsil stained with neat supernatant from T05 hybridoma, detection using Dako Envision kit, 20x power image