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mAbs Against HUMAN Protein

CR1

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:To5
PROTEIN:Complement Receptor Type 1 (CR1, CD35)
PROTEIN WEB:https://www.omim.org/entry/120620
ANTIGEN USED:C3b receptor-enriched precipitates from tonsil cell membranes
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IF WB

DESCRIPTION

CR1 is found on many leucocytes and binds C3b and C4b. To5 may be used in studies of immune complex mediated glomerulopathies.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Immunology. 1982 Apr;45(4):645-53.
Human complement (C3b) receptors defined by a mouse monoclonal antibody.
Gerdes J, Naiem M, Mason DY, Stein H.
PMID: 7068171
https://www.ncbi.nlm.nih.gov/pubmed/7068171

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
To5 neat supernatant Citrate Dako Envision Tonsil /
Immunofluorescence
Enlarge image
To5 Tonsil
Tonsil stained with neat supernatant from To5 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
To5 Tonsil
Tonsil stained with neat supernatant from To5 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
To5 Tonsil
Tonsil stained with neat supernatant from T05 hybridoma, detection using Dako Envision kit, 20x power image