EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network homepage

Menu

mAbs Against HUMAN Protein

CR2

STATUS:
CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:21A5
PROTEIN:Complement component (3d/Epstein Barr virus) receptor 2 (CR2, CD21)
PROTEIN WEB:https://www.omim.org/entry/120650
ANTIGEN USED:CD21
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC

Description

CR2 is expressed strongly on mature B cells, follicular dentritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CR2 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CR2 expression is also gradually lost after stimulation of B cells in vitro. CR2 functions as a receptor for C3d, C3dg and iC3b Complement components and for EBV and for IFN alpha. CR2 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B-cell activation. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, CR2 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.

References

CD Guide for CD21 (1989) In Knapp W, et al (eds) Leucocyte Typing IV, Oxford University Press, Oxford, New York and Tokyo, p 1080. ISBN-13: 978-0192618672

Applications

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
21A/5 Neat supernatant Envision /
Immunofluorescence
Enlarge image
21A/5 Tonsil
Tonsil stained with neat supernatant from 21A/5 hybridoma, detection using Dako Envision kit, 20x power image