mAbs Against HUMAN Protein
CR2
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | 21A5 |
| PROTEIN: | Complement component (3d/Epstein Barr virus) receptor 2 (CR2, CD21) |
| PROTEIN WEB: | https://www.omim.org/entry/120650 |
| ANTIGEN USED: | CD21 |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC |
Description
CR2 is expressed strongly on mature B cells, follicular dentritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CR2 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CR2 expression is also gradually lost after stimulation of B cells in vitro. CR2 functions as a receptor for C3d, C3dg and iC3b Complement components and for EBV and for IFN alpha. CR2 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B-cell activation. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, CR2 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.
References
CD Guide for CD21 (1989) In Knapp W, et al (eds) Leucocyte Typing IV, Oxford University Press, Oxford, New York and Tokyo, p 1080. ISBN-13: 978-0192618672
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 21A/5 | Neat | supernatant | Envision | / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- 21A/5 Tonsil
- Tonsil stained with neat supernatant from 21A/5 hybridoma, detection using Dako Envision kit, 20x power image