mAbs Against HUMAN Protein
Cytokeratin
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | K20 |
| PROTEIN: | Cytokeratins of 46, 48, 50, 55, 56, 56.5, 59, 61, 62, 64, 65, 66 and 67 kDa |
| ANTIGEN USED: | Hair keratin |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG2a Kappa |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | IHC WB |
Description
Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles.
Publication describing antibody characterization/validation
Histopathology. 1985 Aug;9(8):825-40.
The characterization of two monoclonal anti-keratin antibodies and their use in the study of epithelial disorders.
Pulford KA, Gatter KC, Mason DY.
PMID: 2414204
https://www.ncbi.nlm.nih.gov/pubmed/2414204
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| K20 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- K20 Tonsil
- Tonsil stained with neat supernatant from K20 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- K20 Tonsil
- Tonsil stained with neat supernatant from K20 hybridoma, detection using Dako Envision kit, 20x power image