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mAbs Against HUMAN Protein

Cytokeratin

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:K20
PROTEIN:Cytokeratins of 46, 48, 50, 55, 56, 56.5, 59, 61, 62, 64, 65, 66 and 67 kDa
ANTIGEN USED:Hair keratin
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG2a Kappa
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC WB

DESCRIPTION

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Histopathology. 1985 Aug;9(8):825-40.
The characterization of two monoclonal anti-keratin antibodies and their use in the study of epithelial disorders.
Pulford KA, Gatter KC, Mason DY.
PMID: 2414204
https://www.ncbi.nlm.nih.gov/pubmed/2414204

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
K20 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
K20 Tonsil
Tonsil stained with neat supernatant from K20 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
K20 Tonsil
Tonsil stained with neat supernatant from K20 hybridoma, detection using Dako Envision kit, 20x power image