mAbs Against HUMAN Protein
FCGR2
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| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
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| TYPE: | mouse anti human |
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| CLONE NAME: | 2ZC115 |
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| PROTEIN: | Fc gamma Recptor II (FCGR2, CD32) |
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| PROTEIN WEB: | https://www.omim.org/entry/604590 |
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| ANTIGEN USED: | Hairy cell leukaemia cells |
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| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
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| ISOTYPE: | IgG2a |
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| SPECIES REACTIVITY: | Human |
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| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
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| COMMERCIALIZED BY: | Ximbio.com |
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| APP RECOMMENDED: | IHC |
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Description
Human Fc gamma receptor II (CD32) exists in at least six isoforms originating from three different genes (Fc gamma RII A, B, and C). The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses. It is expressed by Monocytes, granulocytes, B cells, eosinophils.
Applications
IHC Techniques
| IHC Techniques |
Clone |
Dilution |
Antibody concentration |
Antigen retrieval method |
Visualization kit |
+/- control |
Protein localization |
Positivity in other species |
Protocol |
| Frozen tissue and cytospins |
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| Paraffin tissue |
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| 2ZC115 |
neat |
supernatant |
Tris EDTA |
Dako Envision |
Tonsil / |
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| Immunofluorescence |
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Enlarge image
- 2ZC115 Tonsil
- Tonsil stained with neat supernatant from 2ZC115 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- 2ZC115 Tonsil
- Tonsil stained with neat supernatant from 2ZC115 hybridoma, detection using Dako Envision kit, 10x power image
