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mAbs Against HUMAN Protein

FCGR3

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:DJ130c
PROTEIN:Fc gamma receptor III
ANTIGEN USED:CD16
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IP WB

DESCRIPTION

FCGR3 mediates phagocytosis and antibody-dependent cell-mediated cytotoxicity. It is expressed on neutorphils, NK cells and macrophages

REFERENCES

Schmidt RE (1993) CD16 cluster workshop report. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 805-806

J Immunol. 1998 Sep 1;161(5):2574-9.
Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils.
Zhu X1, Hamann KJ, Muñoz NM, Rubio N, Mayer D, Hernrreiter A, Leff AR.
PMID: 9725258
https://www.ncbi.nlm.nih.gov/pubmed/9725258

J Leukoc Biol. 1999 Sep;66(3):429-36.
Synovial PMN show a coordinated up-regulation of CD66 molecules.
Hönig M1, Peter HH, Jantscheff P, Grunert F.
PMID: 10496313
https://www.ncbi.nlm.nih.gov/pubmed/10496313

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
DJ130C neat supernatant Citrate Dako Envision Tonsil /
Immunofluorescence
Enlarge image
DJ130C Tonsil
Tonsil stained with neat supernatant from DJ130c hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
DJ130C Tonsil
Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
DJ130C Tonsil
Tonsil stained with neat supernatant from DJ130C hybridoma, detection using Dako Envision kit, 20x power image