mAbs Against HUMAN Protein
FCGR3
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | DJ130c |
| PROTEIN: | Fc gamma receptor III |
| ANTIGEN USED: | CD16 |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC IP WB |
Description
FCGR3 mediates phagocytosis and antibody-dependent cell-mediated cytotoxicity. It is expressed on neutorphils, NK cells and macrophages
References
Schmidt RE (1993) CD16 cluster workshop report. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 805-806
J Immunol. 1998 Sep 1;161(5):2574-9.
Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils.
Zhu X1, Hamann KJ, Muñoz NM, Rubio N, Mayer D, Hernrreiter A, Leff AR.
PMID: 9725258
https://www.ncbi.nlm.nih.gov/pubmed/9725258
J Leukoc Biol. 1999 Sep;66(3):429-36.
Synovial PMN show a coordinated up-regulation of CD66 molecules.
Hönig M1, Peter HH, Jantscheff P, Grunert F.
PMID: 10496313
https://www.ncbi.nlm.nih.gov/pubmed/10496313
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| DJ130C | neat | supernatant | Citrate | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- DJ130C Tonsil
- Tonsil stained with neat supernatant from DJ130c hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- DJ130C Tonsil
- Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- DJ130C Tonsil
- Tonsil stained with neat supernatant from DJ130C hybridoma, detection using Dako Envision kit, 20x power image