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mAbs Against HUMAN Protein

HLADR

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:CR3/43
PROTEIN:Human Leukocyte Antigen DR (MHC class II)
PROTEIN WEB:https://www.omim.org/entry/142857
ANTIGEN USED:Tonsil cells
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC WB

DESCRIPTION

Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). CR3/43 may be used for HLA typing.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Naiem M, Gerdes J, Abdulaziz Z, Nash JRG, Stein H, Mason DY. Production of monoclonal antibodies for the immuno histological analysis of human lymphoma. In: Knapp W, et al, eds. Leukaemia markers. New York: Academic Press,1981:117-25.

REFERENCES

J Clin Pathol. 1982 Nov;35(11):1253-67.
Use of monoclonal antibodies for the histopathological diagnosis of human malignancy.
Gatter KC, Abdulaziz Z, Beverley P, Corvalan JR, Ford C, Lane EB, Mota M, Nash JR, Pulford K, Stein H, Taylor-Papadimitriou J, Woodhouse C, Mason DY.
PMID: 6183295
https://www.ncbi.nlm.nih.gov/pubmed/6183295

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
CR3/43 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
CR3/43 Tonsil
Tonsil stained with neat supernatant from CR3/43 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
CR3/43 Tonsil
Tonsil stained with neat supernatant from CR3/43 hybridoma, detection using Dako Envision kit, 20x power image