mAbs Against HUMAN Protein
HLADRCD74
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | By2 |
| PROTEIN: | Human Leukocyte Antigen-DR locus (HLA-DR) associated invariant chain (CD74) |
| PROTEIN WEB: | https://www.omim.org/entry/142790 |
| ANTIGEN USED: | B cell lymphoma cells |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC IF IP WB |
Description
HLA-DR-associated invariant chain (CD74) plays a role in antigen presentation. In addition to its expression on antigen-presenting cells, CD74 is expressed by carcinomas of renal, lung, gastric, and thymic origin and by certain sarcomas. The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases. It has also been shown to have value as a gastric carcinoma marker.
Publication describing antibody characterization/validation
Jones M et al. 1995. Workshop mAb B029 (By2) - a new B-cell marker for use in diagnostic pathology. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 710-11. ISBN-13: 978-0198577980
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| BY2 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- BY2 Tonsil
- Tonsil stained with neat supernatant from BY2 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- BY2 Tonsil
- Tonsil stained with neat supernatant from BY2 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- BY2 Tonsil
- Tonsil stained with neat supernatant from BY2 hybridoma, detection using Dako Envision kit, 20x power image