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mAbs Against HUMAN Protein

HLADRCD74

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:By2
PROTEIN:Human Leukocyte Antigen-DR locus (HLA-DR) associated invariant chain (CD74)
PROTEIN WEB:https://www.omim.org/entry/142790
ANTIGEN USED:B cell lymphoma cells
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IF IP WB

DESCRIPTION

HLA-DR-associated invariant chain (CD74) plays a role in antigen presentation. In addition to its expression on antigen-presenting cells, CD74 is expressed by carcinomas of renal, lung, gastric, and thymic origin and by certain sarcomas. The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases. It has also been shown to have value as a gastric carcinoma marker.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Jones M et al. 1995. Workshop mAb B029 (By2) - a new B-cell marker for use in diagnostic pathology. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 710-11. ISBN-13: 978-0198577980

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
BY2 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
BY2 Tonsil
Tonsil stained with neat supernatant from BY2 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
BY2 Tonsil
Tonsil stained with neat supernatant from BY2 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
BY2 Tonsil
Tonsil stained with neat supernatant from BY2 hybridoma, detection using Dako Envision kit, 20x power image