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mAbs Against HUMAN Protein

TOX/TOX1

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:Rat anti human
CLONE NAME:NAN448B
PROTEIN:Thymocyte selection-associated high mobility group box protein (TOX)
PROTEIN WEB:https://www.uniprot.org/uniprot/O94900
ANTIGEN USED:His-TOX (1-250aa) recombinant protein
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
NAN448 Neat Supernatant / nuclear
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
NAN448 1:60 Supernatant Leica Tonsil / nuclear Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
NAN448 1:50 Supernatant Tris-EDTA Tonsil / nuclear Available
Enlarge image
NAN448A in transfected cells.
To confirm that NAN448B mAb recognized human TOX protein, immunohistochemistry on frozen cytospins preparations of human TOX expressed in Hek293T was performed. HEK-TOX2 transfected cells were used to test cross-reactivity. Anti-myc and anti-GFP antibodies were used as positive controls.
Enlarge image
NAN448 in human paraffin tissues.
Enlarge image
NAN448B antibody can be used to detect TOX protein in paraffin embedded tissue samples by IF.
Tissue: human tonsil
TOX in red
KI67 in green
DAPI in blue
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
NAN448 Neat supernatant / 57kDa 57kDa Available
Immunoprecipitation
Enlarge image
NAN448 in Western blotting
Predicted molecular weight: 57kDa
Observed molecular weight: 57kDa

Lanes
Lane 1 Hek-TOX transfected cells (10ug) (+)
Lane 2 Hek-TOX2 transfected cells (10ug) (-)
Lane 3 YT cell line (100ug) (-)
Lane 4 Human tonsil (100ug) (+)
Lane 5 K562 cell line (100ug) (-)