mAbs Against HUMAN Protein
TOX2
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | Rat monoclonal |
| CLONE NAME: | SHAW464B |
| PROTEIN: | TOX high mobility group box family member 2 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q96NM4 |
| ANTIGEN USED: | hTOX2-HIS (Fragment expressed: 35-120aa) |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgM |
| SPECIES REACTIVITY: | Human and mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Putative transcriptional activator involved in the hypothalamo-pituitary-gonadal system.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SHAW464B | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SHAW464B | 1:30 | supernatant | Tris-EDTA | Novolink | Tonsil / | Available | ||
| Immunofluorescence | |||||||||
Enlarge image
- SHAW464B mAb is able to detect human TOX2 protein in immunocytochemistry
- To confirm that SHAW464B mAb recognizes human TOX2 protein and it does not cross react with TOX, TOX3 and TOX4 family members, immunocytochemistry on frozen cytospins preparations of human TOX, TOX2, TOX3 and TOX4 expressed in HEK293T was performed. Anti-MYC and anti-GFP Abs were used as a positive control.
Enlarge image
- SHAW464B antibody can be used to detect TOX2 protein in human paraffin tissues.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | SHAW464B | Neat | supernatant | MOLT4 cell line / | 52kDa | 52kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- SHAW464B mAb is able to detect human TOX2 protein by WB.
- LANES
Lane 1 HEK-TOX2-GFP (34KDA) (20ug) (+)
Lane 2 HEK-TOX1 (20ug) (-)
Lane 3 MOLT4 cell line (100ug) (+)
Lane 4 MOLT4 TOX2 KO (100ug) (-)