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mAbs Against HUMAN Protein

TOX2

STATUS:Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:Rat monoclonal
CLONE NAME:SHAW464B
PROTEIN:TOX high mobility group box family member 2
PROTEIN WEB:https://www.uniprot.org/uniprot/Q96NM4
ANTIGEN USED:hTOX2-HIS (Fragment expressed: 35-120aa)
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgM
SPECIES REACTIVITY:Human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

Description

Putative transcriptional activator involved in the hypothalamo-pituitary-gonadal system.

Applications

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
SHAW464B Neat supernatant /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
SHAW464B 1:30 supernatant Tris-EDTA Novolink Tonsil / Available
Immunofluorescence
Enlarge image
SHAW464B mAb is able to detect human TOX2 protein in immunocytochemistry
To confirm that SHAW464B mAb recognizes human TOX2 protein and it does not cross react with TOX, TOX3 and TOX4 family members, immunocytochemistry on frozen cytospins preparations of human TOX, TOX2, TOX3 and TOX4 expressed in HEK293T was performed. Anti-MYC and anti-GFP Abs were used as a positive control.
Enlarge image
SHAW464B antibody can be used to detect TOX2 protein in human paraffin tissues.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
SHAW464B Neat supernatant MOLT4 cell line / 52kDa 52kDa Available
Immunoprecipitation
Enlarge image
SHAW464B mAb is able to detect human TOX2 protein by WB.
LANES

Lane 1 HEK-TOX2-GFP (34KDA) (20ug) (+)
Lane 2 HEK-TOX1 (20ug) (-)
Lane 3 MOLT4 cell line (100ug) (+)
Lane 4 MOLT4 TOX2 KO (100ug) (-)