mAbs Against HUMAN Protein
BCL2
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | bcl-2/90 |
| PROTEIN: | B-cell lymphoma 2 |
| PROTEIN WEB: | https://www.omim.org/entry/151430 |
| ANTIGEN USED: | Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | IHC WB |
Description
The pro-survival protein BCL2 derives its name from B-cell lymphoma 2, as it is the second member of a range of proteins initially described in chromosomal translocations involving chromosomes 14 and 18 in follicular lymphomas. Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments.
Publication describing antibody characterization/validation
Am J Pathol. 1990 Aug;137(2):225-32.
Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation.
Pezzella F1, Tse AG, Cordell JL, Pulford KA, Gatter KC, Mason DY.
PMID: 2201196
https://www.ncbi.nlm.nih.gov/pubmed/2201196
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| bcl-2/90 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- bcl-2/90 Tonsil
- Tonsil stained with neat supernatant from bcl-2/90 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- bcl-2/90 Tonsil
- Tonsil stained with neat supernatant from bcl-2/90 hybridoma, detection using Dako Envision kit, 20x power image