Protocols
Monoclonal Antibody Production Process
The monoclonal antibodies production protocol below has been generated by Jackie Cordell, Lorena Maestre and Karen Pulford, and reviewed by the rest of EuroMAbNet members. This is an excellent and easy to follow guide that will allow you to reproduce all the steps (immunization, hybridoma production, screening , cloning , expanding and freezing down hybridomas, and antibody validation) required for the production of a high quality monoclonal antibodies.
- Antigen
- Immunization
- Antibody producing B cells isolated from the mouse spleen
- PEG
- Cultured myeloma cells
- Hybridoma production
- Screening
- ELISA
- ICC in frozen tissue and cytospin preparation
- Flow cytometry
- Cloning technique
- Monoclonal antibodies produced
- Expanding and freezing down the hybridomas
- Antibody validation
Others related protocols
- Flow cytometry
- Immunofluorescence protocol for culture cells
- Odyssey Western Blotting (OdWB)
- Western Blotting (WB)
- Immunological Screening of bacteriophage l expression libraries
- Production of large quantities of monoclonal antibodies
- BOND-MAX automated immunohistochemistry
- Paraffin-embedded cell line pellet preparation
- Immunofluorescence staining
- Immunoprecipitation
- Transient transfection protocol for HEK293T cells
- Cytospin preparation technique
- PDL-1 mAb IHC-P in mouse tissues