mAbs Against HUMAN Protein
BrdU
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | BU20a |
| PROTEIN: | Bromodeoxyuridine (BrdU) |
| ANTIGEN USED: | Bromodeoxyuridine conjugated to BSA |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC IF |
Description
BrdU is an analogue of thymidine and is taken up into the DNA of proliferating cells. Bu20a works well in immunohistochemistry to identify cells in tissue sections or cytospin preparations that have incorporated BrdU. It is also useful to identify cells in S phase during FACS analysis.
Publication describing antibody characterization/validation
J Histochem Cytochem. 1989 Oct;37(10):1517-27.
Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.
Magaud JP1, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY.
PMID: 2476478
https://www.ncbi.nlm.nih.gov/pubmed/2476478