mAbs Against HUMAN Protein
CD19
| STATUS: | |
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| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
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| TYPE: | mouse anti human |
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| CLONE NAME: | LC-1/32/A10 |
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| PROTEIN: | CD19 |
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| PROTEIN WEB: | https://www.omim.org/entry/107265 |
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| ANTIGEN USED: | A synthetic peptide, corresponding to the C-Terminus of CD19. |
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| FUSION PARTNER: | NS0 |
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| ISOTYPE: | IgG1 kappa |
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| SPECIES REACTIVITY: | Human |
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| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
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| COMMERCIALIZED BY: | Ximbio.com |
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| APP RECOMMENDED: | IHC WB |
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Description
CD19 is a cell surface molecule expressed on B lymphocytes and follicular dendritic cells. It is expressed by most pre-B cells and by the majority of non-T-cell acute lymphocytic leukaemias and B-cell type chronic lymphocytic leukaemias. CD19 assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Applications
IHC Techniques
| IHC Techniques |
Clone |
Dilution |
Antibody concentration |
Antigen retrieval method |
Visualization kit |
+/- control |
Protein localization |
Positivity in other species |
Protocol |
| Frozen tissue and cytospins |
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| Paraffin tissue |
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| 32/A10 |
neat |
supernatant |
Tris EDTA |
Dako Envision |
Tonsil / |
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|
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| Immunofluorescence |
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Enlarge image
- LC-1/32/A10 Tonsil
- Tonsil stained with neat supernatant from LC-1/32/A10 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- LC-1/32/A10
- Tonsil stained with neat supernatant from LC-1/32/A10 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- LC-1/32/A10
- Tonsil stained with neat supernatant from LC-1/32/A10 hybridoma, detection using Dako Envision kit, 20x power image
