mAbs Against HUMAN Protein
CD79b
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | B29/123 |
| PROTEIN: | CD79b |
| PROTEIN WEB: | https://www.omim.org/entry/147245 |
| ANTIGEN USED: | Synthetic peptide representing the c-terminus from residue 215 of the murine B29 polypeptide. |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG2b |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | IHC WB |
Description
The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling.
Publication describing antibody characterization/validation
Eur J Immunol. 1992 Oct;22(10):2753-6.
The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation.
Mason DY1, van Noesel CJ, Cordell JL, Comans-Bitter WM, Micklem K, Tse AG, van Lier RA, van Dongen JJ.
PMID: 1396979
https://www.ncbi.nlm.nih.gov/pubmed/1396979
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| B29/123 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- B29/123 Tonsil
- Tonsil stained with neat supernatant from B29/123 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
- B29/123 Tonsil
- Tonsil stained with neat supernatant from B29/123 hybridoma, detection using Dako Envision kit, 20x power image