mAbs Against HUMAN Protein
|CONTACT INFORMATION:||University of Oxford marketed by Ximbio.com|
|TYPE:||mouse anti human|
|ANTIGEN USED:||Synthetic peptide representing the c-terminus from residue 215 of the murine B29 polypeptide.|
|PREPARATION AND STORAGE:||Aliquot and store at 4C. Do not freeze|
|APP RECOMMENDED:||IHC WB|
The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling.
PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION
Eur J Immunol. 1992 Oct;22(10):2753-6.
The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation.
Mason DY1, van Noesel CJ, Cordell JL, Comans-Bitter WM, Micklem K, Tse AG, van Lier RA, van Dongen JJ.
|IHC Techniques||Clone||Dilution||Antibody concentration||Antigen retrieval method||Visualization kit||+/- control||Protein localization||Positivity in other species||Protocol|
|Frozen tissue and cytospins|
|B29/123||neat||supernatant||Tris EDTA||Dako Envision||Tonsil /|
- B29/123 Tonsil
- Tonsil stained with neat supernatant from B29/123 hybridoma, detection using Dako Envision kit, 4x power image
- B29/123 Tonsil
- Tonsil stained with neat supernatant from B29/123 hybridoma, detection using Dako Envision kit, 20x power image