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mAbs Against HUMAN Protein

CD79b

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:B29/123
PROTEIN:CD79b
PROTEIN WEB:https://www.omim.org/entry/147245
ANTIGEN USED:Synthetic peptide representing the c-terminus from residue 215 of the murine B29 polypeptide.
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG2b
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC WB

DESCRIPTION

The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Eur J Immunol. 1992 Oct;22(10):2753-6.
The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation.
Mason DY1, van Noesel CJ, Cordell JL, Comans-Bitter WM, Micklem K, Tse AG, van Lier RA, van Dongen JJ.
PMID: 1396979
https://www.ncbi.nlm.nih.gov/pubmed/1396979

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
B29/123 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
B29/123 Tonsil
Tonsil stained with neat supernatant from B29/123 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
B29/123 Tonsil
Tonsil stained with neat supernatant from B29/123 hybridoma, detection using Dako Envision kit, 20x power image