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mAbs Against HUMAN Protein

CDw75

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:ZB55
PROTEIN:FACS IHC IF
PROTEIN WEB:https://www.omim.org/entry/109675
ANTIGEN USED:CD32 peptides
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgM
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IF

DESCRIPTION

CDw75 is a human leukocyte cell-surface antigen expressed in mature and activated B cells but not in B cells at earlier stages of development or in plasma cells. ZB55 can be used for studying germinal centre-derived B cell lymphomas.

Please note that CD32 peptides were used as the immunogen although the hybridoma ultimately selected produces antibodies specific to CDw75.

REFERENCES

Tedder TF. et al.1995. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 494. ISBN-13: 978-0198577980

Pulford K. et al. 1996. Immunocytochemical distribution of the CDw75 antigen. Tissue Antigens. Abstract. 48:371-85. DOI: 10.1111/j.1399-0039.1996.tb02657.x

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
ZB55 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
ZB55 Tonsil
Tonsil stained with neat supernatant from ZB55 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
ZB55 Tonsil
Tonsil stained with neat supernatant from ZB55 hybridoma, detection using Dako Envision kit, 20x power image