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mAbs Against HUMAN Protein

EMA

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:E29
PROTEIN:Epithelial Membrane Antigen (EMA)
PROTEIN WEB:https://www.omim.org/entry/158340
ANTIGEN USED:Human milk fat globule membrane preparation
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG2a
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC WB

DESCRIPTION

Glycoproteins isolated from human milk fat globule membranes, designated epithelial membrane antigen (EMA), have been detected immunohistochemically in most nonneoplastic epithelia and are potentially a highly effective marker for establishing the epithelial nature of neoplastic cells.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Br J Cancer. 1985 Sep;52(3):347-54.
Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry.
Cordell J, Richardson TC, Pulford KA, Ghosh AK, Gatter KC, Heyderman E, Mason DY.
PMID: 3899155
https://www.ncbi.nlm.nih.gov/pubmed/3899155

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
E29 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
E29 Tonsil
Tonsil stained with neat supernatant from E29 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
E29 Tonsil
Tonsil stained with neat supernatant from E29 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
E29 Tonsil
Tonsil stained with neat supernatant from E29 hybridoma, detection using Dako Envision kit, 20x power image