mAbs Against HUMAN Protein
ICAM3
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | CG106 |
| PROTEIN: | Intercellular Adhesion Molecule 3, CD50 |
| PROTEIN WEB: | https://www.omim.org/entry/146631 |
| ANTIGEN USED: | T-lymphoblastic cells |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC IP WB |
Description
ICAM-3 is the major ligand for LFA-1 (CD11a/CD18) in the resting state. ICAM-3 may play a key role in initiating immune responses.
Publication describing antibody characterization/validation
J Clin Pathol. 1994 Feb;47(2):143-7.
Cellular distribution of human leucocyte adhesion molecule ICAM-3.
Cordell JL1, Pulford K, Turley H, Jones M, Micklem K, Doussis IA, Tyler X, Mayne K, Gatter KC, Mason DY.
PMID: 8132828
https://www.ncbi.nlm.nih.gov/pubmed/8132828
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| CG106 | neat | supernatant | Citrate | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- CG106 Tonsil
- Tonsil stained with neat supernatant from CG106 hybridoma, detection using Dako Envision kit, 20x power image