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mAbs Against HUMAN Protein

Leu1

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:CD5/54/F6
PROTEIN:Leu1 (CD5)
PROTEIN WEB:https://www.omim.org/entry/153340
ANTIGEN USED:Peptide from the intracellular region of CD5 (SSMQPDNSSDSDYDLHGAQRL)
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human, Bovine, Chicken, Horse, Guinea Pig, Opossum, Pig, Primate, Rat and Rabbit
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC WB

DESCRIPTION

CD5 is a surface glycoprotein present on peripheral T cells (>95%) and B-cell chronic lymphocytic leukaemia cells that binds CD72. CD5 has utility for the monitoring of T-cell numbers in peripheral blood and the identification of leukaemias of T cell origin. Absence of the CD5 marker on childhood ALL cells is associated with a more favourable prognosis.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

J Immunol. 1993 Jun 15;150(12):5429-35.
Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
Jones M1, Cordell JL, Beyers AD, Tse AG, Mason DY.
PMID: 8515069
https://www.ncbi.nlm.nih.gov/pubmed/8515069

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
CD5/54/F6 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
CD5/54/F6 Tonsil
Tonsil stained with neat supernatant from CD5/54/F6 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
CD5/54/F6 Tonsil
Tonsil stained with neat supernatant from CD5/54/F6 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
CD5/54/F6
Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 10x power image