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mAbs Against HUMAN Protein

PECAM1

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:JC70
PROTEIN:PECAM1 (CD31)
PROTEIN WEB:https://www.omim.org/entry/173445
ANTIGEN USED:Cell membrane preparation from the spleen of a patient with hairy cell leukaemia
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IF WB

DESCRIPTION

PECAM1 (CD31) is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, particular T-cell subsets, and is also a major constituent of the endothelial cell intercellular junction. PECAM1 is implicated in several functions, including transendothelial migration of leukocytes, angiogenesis, and integrin activation. JC70 is a good marker of endothelial cells in paraffin sections.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

J Clin Pathol. 1990 Sep;43(9):752-7.
JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections.
Parums DV1, Cordell JL, Micklem K, Heryet AR, Gatter KC, Mason DY.
PMID: 2212067
https://www.ncbi.nlm.nih.gov/pubmed/2212067

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
JC70 neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
JC70 Tonsil
Tonsil stained with neat supernatant from JC70 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
JC70 Tonsil
Tonsil stained with neat supernatant from JC70 hybridoma, detection using Dako Envision kit, 20x power image