mAbs Against HUMAN Protein
PECAM1
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | JC70 |
| PROTEIN: | PECAM1 (CD31) |
| PROTEIN WEB: | https://www.omim.org/entry/173445 |
| ANTIGEN USED: | Cell membrane preparation from the spleen of a patient with hairy cell leukaemia |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC IF WB |
Description
PECAM1 (CD31) is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, particular T-cell subsets, and is also a major constituent of the endothelial cell intercellular junction. PECAM1 is implicated in several functions, including transendothelial migration of leukocytes, angiogenesis, and integrin activation. JC70 is a good marker of endothelial cells in paraffin sections.
Publication describing antibody characterization/validation
J Clin Pathol. 1990 Sep;43(9):752-7.
JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections.
Parums DV1, Cordell JL, Micklem K, Heryet AR, Gatter KC, Mason DY.
PMID: 2212067
https://www.ncbi.nlm.nih.gov/pubmed/2212067
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| JC70 | neat | supernatant | Tris EDTA | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- JC70 Tonsil
- Tonsil stained with neat supernatant from JC70 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- JC70 Tonsil
- Tonsil stained with neat supernatant from JC70 hybridoma, detection using Dako Envision kit, 20x power image