EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network

☰ Menu

mAbs Against HUMAN Protein

Von Willebrand Factor

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:F8/44/20
PROTEIN:Von Willebrand factor (VWF)
PROTEIN WEB:https://www.omim.org/entry/613160
ANTIGEN USED:Von Willebrand factor isolated from human plasma
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:ELISA IHC WB

DESCRIPTION

Von Willebrand factor (vWF) mediates platelet adhesion to injured endothelium, the first step in hemostasis, and helps maintain factor VIII levels. When vWF is deficient, patients have a bleeding disorder called von Willebrand disease (vWD).

REFERENCES

Br J Haematol. 1984 Mar;56(3):365-86.
Immunohistological analysis of human bone marrow trephine biopsies using monoclonal antibodies.
Falini B, Martelli MF, Tarallo F, Moir DJ, Cordell JL, Gatter KC, Loreti G, Stein H, Mason DY.
PMID: 6365152
https://www.ncbi.nlm.nih.gov/pubmed/6365152

J Immunol Methods. 1982;50(2):145-60.
The value of immunohistological screening in the production of monoclonal antibodies.
Naiem M, Gerdes J, Abdulaziz Z, Sunderland CA, Allington MJ, Stein H, Mason DY.
PMID: 6806388
https://www.ncbi.nlm.nih.gov/pubmed/6806388

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
F8/44/20 neat supernatant Citrate Dako Envision Tonsil /
Immunofluorescence
Enlarge image
F8/44/20 Tonsil
Tonsil stained with neat supernatant from F8/44/20 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
F8/44/20 Tonsil
Tonsil stained with neat supernatant from F8/44/20 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
F8/44/20 Tonsil
Tonsil stained with neat supernatant from F8/44/20 hybridoma, detection using Dako Envision kit, 20x power image