mAbs Against HUMAN Protein
Von Willebrand Factor
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | F8/44/20 |
| PROTEIN: | Von Willebrand factor (VWF) |
| PROTEIN WEB: | https://www.omim.org/entry/613160 |
| ANTIGEN USED: | Von Willebrand factor isolated from human plasma |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | ELISA IHC WB |
Description
Von Willebrand factor (vWF) mediates platelet adhesion to injured endothelium, the first step in hemostasis, and helps maintain factor VIII levels. When vWF is deficient, patients have a bleeding disorder called von Willebrand disease (vWD).
References
Br J Haematol. 1984 Mar;56(3):365-86.
Immunohistological analysis of human bone marrow trephine biopsies using monoclonal antibodies.
Falini B, Martelli MF, Tarallo F, Moir DJ, Cordell JL, Gatter KC, Loreti G, Stein H, Mason DY.
PMID: 6365152
https://www.ncbi.nlm.nih.gov/pubmed/6365152
J Immunol Methods. 1982;50(2):145-60.
The value of immunohistological screening in the production of monoclonal antibodies.
Naiem M, Gerdes J, Abdulaziz Z, Sunderland CA, Allington MJ, Stein H, Mason DY.
PMID: 6806388
https://www.ncbi.nlm.nih.gov/pubmed/6806388
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| F8/44/20 | neat | supernatant | Citrate | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- F8/44/20 Tonsil
- Tonsil stained with neat supernatant from F8/44/20 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- F8/44/20 Tonsil
- Tonsil stained with neat supernatant from F8/44/20 hybridoma, detection using Dako Envision kit, 10x power image
Enlarge image
- F8/44/20 Tonsil
- Tonsil stained with neat supernatant from F8/44/20 hybridoma, detection using Dako Envision kit, 20x power image