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mAbs Against MOUSE Protein

NANOG

CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
TYPE:rat anti mouse
CLONE NAME:NF1A
PROTEIN:Mouse full length NANOG (Homeo box transcription factor nanog) protein
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/110625918
ANTIGEN USED:HIS-mNANOG full length protein
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cell
ISOTYPE:IgG2a
SPECIES REACTIVITY:mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG is thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
NF1A neat Supernatant Discovery Xt CC1 OmniMap Ventana Teratoma / Lymph node Nuclear
Immunofluorescence
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NF1A mAb in transfected cells
Nuclear staining on frozen cytospin preparations of transfected HEK293T/mNANOG cells using antibody NF1A. HEK293T/FGFR1 transfected cells were used as negative control. Anti-v5 was used as positive control.
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NANOG (NF1A) staining in mouse paraffin section.
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Nanog (NF1A) expression on mouse paraffin sections.
Liver from Nanog transgenic mice in which the expression of Nanog is induced by tetracycline (teton sysyem). A without tetracycline , B with low dose of tetracycline and C high doses of tetracycline.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
NF1A Undiluted Supernatant NTERA2 cell line / 34kDa 34kDa Available
Immunoprecipitation
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Western Blotting of mNANOG (NF1A) using different cell lines.
Lane 1 Ces-v6.4 knbf1gamma (15ug) (+)
Lane 2 Ntera2 cell line (150ug) (+)
Lane 3 Ces-BT12kn bf1 gamma (15ug) (-)
Lane 4 Hek-mNANOG-v5 (20ug) (+)