mAbs Against MOUSE Protein
NANOG
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| TYPE: | rat anti mouse |
| CLONE NAME: | NF1A |
| PROTEIN: | Mouse full length NANOG (Homeo box transcription factor nanog) protein |
| PROTEIN WEB: | http://www.ncbi.nlm.nih.gov/protein/110625918 |
| ANTIGEN USED: | HIS-mNANOG full length protein |
| FUSION PARTNER: | myeloma p3-NS1/Ag4-1 (NS1) cell |
| ISOTYPE: | IgG2a |
| SPECIES REACTIVITY: | mouse |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG is thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NF1A | neat | Supernatant | Discovery Xt CC1 OmniMap | Ventana | Teratoma / Lymph node | Nuclear | ||
| Immunofluorescence | |||||||||
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- NF1A mAb in transfected cells
- Nuclear staining on frozen cytospin preparations of transfected HEK293T/mNANOG cells using antibody NF1A. HEK293T/FGFR1 transfected cells were used as negative control. Anti-v5 was used as positive control.
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- NANOG (NF1A) staining in mouse paraffin section.
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- Nanog (NF1A) expression on mouse paraffin sections.
- Liver from Nanog transgenic mice in which the expression of Nanog is induced by tetracycline (teton sysyem). A without tetracycline , B with low dose of tetracycline and C high doses of tetracycline.
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NF1A | Undiluted | Supernatant | NTERA2 cell line / | 34kDa | 34kDa | Available | |
| Immunoprecipitation | ||||||||
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- Western Blotting of mNANOG (NF1A) using different cell lines.
- Lane 1 Ces-v6.4 knbf1gamma (15ug) (+)
Lane 2 Ntera2 cell line (150ug) (+)
Lane 3 Ces-BT12kn bf1 gamma (15ug) (-)
Lane 4 Hek-mNANOG-v5 (20ug) (+)