Name of the laboratory
- Monoclonal Antibodies Unit
- Centro Nacional de Investigaciones Oncológicas
- Calle Melchor Fernández Almagro 3
- 28029 Madrid, Spain
- 34 912 246 900
Members of the laboratory
- Unit Leader
Giovanna Roncador firstname.lastname@example.org
Lorena Maestre email@example.com
Mar López Colomer firstname.lastname@example.org
Ana Isabel Reyes García email@example.com
Hybridoma technology for the production of monoclonal antibodies represents one of the most relevant methodological advances in biomedicine. The availability of monoclonal antibodies has significantly improved our knowledge about cell biology, opening new possibilities for basic and applied research. Its use in medicine has also improved the diagnostics, prevention and treatment of a large number of diseases.
The Monoclonal Antibodies (mAb) Unit provides CNIO Research Groups with the “à la carte”, generation of mAbs which can then be used as tools to characterise new pathways involved in cancer development. We are highly specialised in mouse and rat monoclonal antibodies production. The Unit also offers mAbs production in gene-inactivated mice, mAb characterisation and validation, medium-scale mAb production and a service of Mycoplasma testing for the cell culture facility.
Mouse specific antibodies: During the latest years, genetic approaches have been extensively used at the CNIO for the functional analysis of genes directly implicated in the process of tumor transformation and progression. The study of mouse models has been hampered by the absence of reliable mouse-specific antibodies to be used on paraffin-embedded tissues. For this reason, and in collaboration with the CNIO Comparative Pathology Unit, we have tested and developed a panel of high quality mouse specific mAbs, able to detect their target molecules on paraffin mouse tissue sections by immunohistochemistry.
Rat mAb: Many protein targets relevant for signal transduction and disease are highly conserved between mice and humans, and can therefore be recognized as self-antigens by a mouse host. To overcome this problem, this year we have made available in our unit a new service of production of mAbs in rats. This will provide CNIO investigators with a powerful tool to study cell biology and cancer related processes in mouse models. The mAbs against mouse protein and produced in rat to date are directed against the following proteins: Beta-Gal, p15,p16,p21,p53, Hes-1and Nanog.
- monoclonal antibody production in mice
- polyclonal antibody production
- cloning technique
- antibody characterization
- development of double and triple immunostaining techniques
- mycoplasma testing
- genetic immunization
- Ramos-Medina R, Montes-Moreno S, Maestre L, Cañamero M, Rodríguez-Pinilla M, Martínez-Torrecuadrada J, Piris MA, Majid A, Dyer MJ, Pulford K, Roncador G (2013). BCL7A protein expression in normal and malignant lymphoid tissues
Br J Haemato 160(1):106-9.
- Montes-Moreno S, Ramos-Medina R, Martínez-López A, Barrionuevo Cornejo C, Parra Cubillos A, Quintana-Truyenque S, Rodriguez Pinilla SM, Pajares R, Sanchez-Verde L, Roncador G, Piris MA (2013). SPIB, a novel immunohistochemical marker for human blastic plasmacytoid dendritic cell neoplasms: characterization of its expression in major haematolymphoid neoplasms.
- Burnichon N, Cascón A, Schiavi F, Morales NP, Comino-Méndez I, Abermil N, Inglada-Pérez L, de Cubas AA, Amar L, Barontini M, de Quirós SB, Bertherat J, Bignon YJ, Blok MJ, Bobisse S, Borrego S, Castellano M, Chanson P, Chiara MD, Corssmit EP, Giacchè M, de Krijger RR, Ercolino T, Girerd X, Gómez-García EB, Gómez-Graña A, Guilhem I, Hes FJ, Honrado E, Korpershoek E, Lenders JW, Letón R, Mensenkamp AR, Merlo A, Mori L, Murat A, Pierre P, Plouin PF, Prodanov T, Quesada-Charneco M, Qin N, Rapizzi E, Raymond V, Reisch N, Roncador G, Ruiz-Ferrer M, Schillo F, Stegmann AP, Suarez C, Taschin E, Timmers HJ, Tops CM, Urioste M, Beuschlein F, Pacak K, Mannelli M, Dahia PL, Opocher G, Eisenhofer G, Gimenez-Roqueplo AP, Robledo M (2012). MAX mutations cause hereditary and sporadic pheochromocytoma and paraganglioma.
Clin Cancer Res 15, 2828-37.
- Patel S, Chen H, Monti L, Gould E, Haralambieva E, Schmid J, Toomey D, Woessmann W, Roncador G, Hatton CS, Liggins AP, Taramelli R, Banham AH, Acquati F, Murphy D, Pulford K (2012). RNASET2 an autoantigen in anaplastic large cell lymphoma identified by protein array analysis.
J Proteomics 18, 5279-92
- Kempf W, Kazakov DV, Cipolat C, Kutzner H, Roncador G, Tomasini D (2012). CD4/CD8 Double Negative Mycosis Fungoides With PD-1 (CD279) Expression-A Disease of Follicular Helper T-Cells?
Am J dermatopathol 7, 757-761
- Martin-Arruti M, Vaquero M, Díaz de Otazu R, Zabalza I, Ballesteros J, Roncador G, García-Orad A (2012). Bcl-2 and BLIMP-1 expression predict worse prognosis in gastric diffuse large B cell lymphoma (DLCBL) while other markers for nodal DLBCL are not useful.
Histopathology 5, 785-92
- Guijarro MV, Vergel M, Marin JJ, Muñoz-Galván S, Ferrer I, Cajal SR, Roncador G, Blanco-Aparicio C, Carnero A (2012). p38α limits the contribution of MAP17 to cancer progression in breast tumors.
- CroatiaStipan JonjicUniversity of Rijeka
- FranceAndré PèlegrinInstitut de Recherche en Cancérologie de Montpellier, IRCM
- FranceArmand BensussanHôpital Saint Louis
- FrancePierre LafayeInstitut Pasteur
- GermanyFriedrich Koch-NolteUniversity Medical Center Hamburg-Eppendorf
- HungaryJohanna SymmonsEnglish & Scientific Consulting Kft. (SCICONS)
- HungaryPeter BaloghClinical Center, University of Pecs
- ItalyFabio MalavasiUniversity of Torino Medical School
- RussiaAlexander FilatovInstitute of Immunology
- RussiaAlexander TaraninInstitute of Molecular and Cellular Biology
- SloveniaVladka Čurin ŠerbecBlood Trasfusion Centre of Slovenia
- SpainGiovanna RoncadorCentro Nacional de Investigaciones Oncológicas
- SpainJosé I. Rodríguez BarbosaEdificio Nuevos Institutos de Investigación
- SpainLeonor KremerCentro Nacional de Biotecnología/CSIC
- SpainNúria PascualNb4D
- SpainPablo EngelMedical School, University of Barcelona
- United KingdomUniversity of OxfordLRF Haemato-oncology Group
- United KingdomVertebrate Antibodies Limited (VAL)
- United KingdomUniversity of OxfordOxFabs
- United KingdomKing's College LondonSt. John's Institute of Dermatology