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Giovanna Roncador

Name of the laboratory

CNIO: Centro Nacional de Investigaciones Oncológicas
Monoclonal Antibodies Unit
Centro Nacional de Investigaciones Oncológicas
Calle Melchor Fernández Almagro 3
28029 Madrid, Spain
34 912 246 900

Members of the laboratory

Members: Monoclonal Antibodies Unit
Unit Leader
Giovanna Roncador groncador@cnio.es
Technicians
Lorena Maestre lmaestre@cnio.es
Ana Isabel Reyes García aireyes@cnio.es
Sherezade Jiménez sjimenez@srv.cnio.es

Laboratory activity

Hybridoma technology for the production of monoclonal antibodies represents one of the most relevant methodological advances in biomedicine. The availability of monoclonal antibodies has significantly improved our knowledge about cell biology, opening new possibilities for basic and applied research. Its use in medicine has also improved the diagnostics, prevention and treatment of a large number of diseases.

The Monoclonal Antibodies (mAb) Unit provides CNIO Research Groups with the “à la carte”, generation of mAbs which can then be used as tools to characterise new pathways involved in cancer development. We are highly specialised in mouse and rat monoclonal antibodies production. The Unit also offers mAbs production in gene-inactivated mice, mAb characterisation and validation, medium-scale mAb production and a service of Mycoplasma testing for the cell culture facility.

Research activities

Mouse specific antibodies: During the latest years, genetic approaches have been extensively used at the CNIO for the functional analysis of genes directly implicated in the process of tumor transformation and progression. The study of mouse models has been hampered by the absence of reliable mouse-specific antibodies to be used on paraffin-embedded tissues. For this reason, and in collaboration with the CNIO Comparative Pathology Unit, we have tested and developed a panel of high quality mouse specific mAbs, able to detect their target molecules on paraffin mouse tissue sections by immunohistochemistry.

Rat mAb: Many protein targets relevant for signal transduction and disease are highly conserved between mice and humans, and can therefore be recognized as self-antigens by a mouse host. To overcome this problem, this year we have made available in our unit a new service of production of mAbs in rats. This will provide CNIO investigators with a powerful tool to study cell biology and cancer related processes in mouse models. The mAbs against mouse protein and produced in rat to date are directed against the following proteins: Beta-Gal, p15,p16,p21,p53, Hes-1and Nanog.

Techniques available

  • monoclonal antibody production in mice and rat
  • polyclonal antibody production
  • recombinant antibody production
  • cloning technique
  • antibody characterization
  • antibody sequencing
  • development of double and triple immunostaining techniques
  • mycoplasma testing
  • genetic immunization

Publications (2021-present)

  • Kanzaria A, Arora S, Naik A, Dhanushkodi N, Ho CC, Kaur N, Zhang J, Ren X, Fromm JR, Shadman M, Smith S, Gopal A, Roncador G, Holland E, Naresh KN. Biomarkers Informed by Single-Cell and Spatial Transcriptomics-Biomarkers for Grade 3 Follicular Lymphoma. Mod Pathol. 2026 Jan 9;39(3):100958. doi: 10.1016/j.modpat.2026.100958. Epub ahead of print. PMID: 41521011.
  • Cirauqui C, Ojeda L, Otano I, Pazos I, Santos A, Garrido-Martín EM, Yagüe P, Ramos-Paradas J, Molina-Pinelo S, Roncador G, Solórzano JL, Muñoz MT, Cozar P, Plaza P, Suárez R, Jiménez M, Moreno R, Rosado A, Gámez P, García-Luján R, Zugazagoitia J, Sweet-Cordero EA, Barbacid M, Carnero A, Ferrer I, Paz-Ares L. Interleukin-11 promotes lung adenocarcinoma tumourigenesis and immune evasion. Clin Transl Med. 2025 Jul;15(7):e70374. doi: 10.1002/ctm2.70374. PMID: 40673604; PMCID: PMC12268789.
  • Ikoma H, Carreras J, Kikuti YY, Miyaoka M, Nagase S, Kondo Y, Ito A, Orita M, Tomita S, Hiraiwa S, Kawada H, Garcia JF, Roncador G, Campo E, Nakamura N. Comparison of the Mutational Profile between BCL2- and BCL6-Rearrangement Positive Follicular Lymphoma. J Mol Diagn. 2025 Aug;27(8):796-807. doi: 10.1016/j.jmoldx.2025.05.002. Epub 2025 Jun 5. PMID: 40482882.
  • Del Rio ML, Nuero-Garcia OM, Roncador G, Garcimartín-Bailon R, Cubria JC, Schneider P, Rodriguez-Barbosa JI. A bioactive soluble recombinant mouse LIGHT promotes effective tumor immune cell infiltration delaying tumor growth. J Mol Med (Berl). 2025 Jul;103(7):867-883. doi: 10.1007/s00109-025-02552-x. Epub 2025 Jun 2. PMID: 40457031; PMCID: PMC12287161.
  • Carreras J, Roncador G, Hamoudi R, Bombi JA, Masugi Y. Intraepithelial Lymphocytes and LAIR1 Expression in Celiac Disease. Biomedicines. 2025 Oct 16;13(10):2526. doi: 10.3390/biomedicines13102526. PMID: 41153806; PMCID: PMC12561610.
  • Carreras J, Roncador G, Hamoudi R. Ulcerative Colitis, LAIR1 and TOX2 Expression, and Colorectal Cancer Deep Learning Image Classification Using Convolutional Neural Networks. Cancers (Basel). 2024 Dec 19;16(24):4230. doi: 10.3390/cancers16244230. PMID: 39766129; PMCID: PMC11674594.
  • Monteagudo M, Calsina B, Salazar-Hidalgo ME, Martínez-Montes ÁM, Piñeiro-Yáñez E, Caleiras E, Martín MC, Rodríguez-Perales S, Letón R, Gil E, Buffet A, Burnichon N, Fernández-Sanromán Á, Díaz-Talavera A, Mellid S, Arroba E, Reglero C, Martínez-Puente N, Roncador G, Del Olmo MI, Corrales PJP, Oliveira CL, Álvarez-Escolá C, Gutiérrez MC, López-Fernández A, García NP, Regojo RM, Díaz LR, Laorden NR, Guadarrama OS, Bechmann N, Beuschlein F, Canu L, Eisenhofer G, Fassnacht M, Nölting S, Quinkler M, Rapizzi E, Remde H, Timmers HJ, Favier J, Gimenez-Roqueplo AP, Rodriguez-Antona C, Currás-Freixes M, Al-Shahrour F, Cascón A, Leandro-García LJ, Montero-Conde C, Robledo M. MAML3-fusions modulate vascular and immune tumour microenvironment and confer high metastatic risk in pheochromocytoma and paraganglioma. Best Pract Res Clin Endocrinol Metab. 2024 Dec;38(6):101931. doi: 10.1016/j.beem.2024.101931. Epub 2024 Aug 29. PMID: 39218714.
  • Menéndez V, Solórzano JL, García-Cosío M, Cereceda L, Díaz E, Estévez M, Roncador G, Vega Z, Montalbán C, Kulasinghe A, García JF. Mapping the Spatial Dynamics of the CD4+ T Cell Spectrum in Classical Hodgkin Lymphoma. Mod Pathol. 2024 Sep;37(9):100551. doi: 10.1016/j.modpat.2024.100551. Epub 2024 Jun 25. PMID: 38936478.
  • Carreras J, Ikoma H, Kikuti YY, Miyaoka M, Hiraiwa S, Tomita S, Kondo Y, Ito A, Nagase S, Miura H, Kawada H, Roncador G, Campo E, Hamoudi R, Nakamura N. Mutational, immune microenvironment, and clinicopathological profiles of diffuse large B-cell lymphoma and follicular lymphoma with BCL6 rearrangement. Virchows Arch. 2024 Apr;484(4):657-676. doi: 10.1007/s00428-024-03774-z. Epub 2024 Mar 11. PMID: 38462571.
  • Felipe I, Martínez-de-Villarreal J, Patel K, Martínez-Torrecuadrada J, Grossmann LD, Roncador G, Cubells M, Farrell A, Kendsersky N, Sabroso-Lasa S, Sancho-Temiño L, Torres K, Martinez D, Perez JM, García F, Pogoriler J, Moreno L, Maris JM, Real FX. BPTF cooperates with MYCN and MYC to link neuroblastoma cell cycle control to epigenetic cellular states. bioRxiv [Preprint]. 2024 Feb 12:2024.02.11.579816. doi: 10.1101/2024.02.11.579816. PMID: 38405949; PMCID: PMC10888818.
  • Roldán-Romero JM, Valdivia C, Santos M, Lanillos J, Maroto P, Anguera G, Calsina B, Martinez-Montes A, Monteagudo M, Mellid S, Leandro-García LJ, Montero-Conde C, Cascón A, Roncador G, Coloma J, Robledo M, Rodriguez-Antona C. Deubiquitinase USP9X loss sensitizes renal cancer cells to mTOR inhibition. Int J Cancer. 2023 Sep 15;153(6):1300-1312. doi: 10.1002/ijc.34575. Epub 2023 Jun 1. PMID: 37260183.
  • Del Rio ML, de Juan CY, Roncador G, Caleiras E, Álvarez-Esteban R, Pérez-Simón JA, Rodriguez-Barbosa JI. Genetic deletion of HVEM in a leukemia B cell line promotes a preferential increase of PD-1- stem cell-like T cells over PD-1+ T cells curbing tumor progression. Front Immunol. 2023 Mar 23;14:1113858. doi: 10.3389/fimmu.2023.1113858. PMID: 37033927; PMCID: PMC10076739.
  • Carreras J, Kikuti YY, Miyaoka M, Hiraiwa S, Tomita S, Ikoma H, Kondo Y, Ito A, Nagase S, Miura H, Roncador G, Colomo L, Hamoudi R, Campo E, Nakamura N. Mutational Profile and Pathological Features of a Case of Interleukin-10 and RGS1-Positive Spindle Cell Variant Diffuse Large B-Cell Lymphoma. Hematol Rep. 2023 Mar 12;15(1):188-200. doi: 10.3390/hematolrep15010020. PMID: 36975733; PMCID: PMC10048669.
  • Larrayoz M, Garcia-Barchino MJ, Celay J, Etxebeste A, Jimenez M, Perez C, Ordoñez R, Cobaleda C, Botta C, Fresquet V, Roa S, Goicoechea I, Maia C, Lasaga M, Chesi M, Bergsagel PL, Larrayoz MJ, Calasanz MJ, Campos-Sanchez E, Martinez-Cano J, Panizo C, Rodriguez-Otero P, Vicent S, Roncador G, Gonzalez P, Takahashi S, Katz SG, Walensky LD, Ruppert SM, Lasater EA, Amann M, Lozano T, Llopiz D, Sarobe P, Lasarte JJ, Planell N, Gomez-Cabrero D, Kudryashova O, Kurilovich A, Revuelta MV, Cerchietti L, Agirre X, San Miguel J, Paiva B, Prosper F, Martinez-Climent JA. Preclinical models for prediction of immunotherapy outcomes and immune evasion mechanisms in genetically heterogeneous multiple myeloma. Nat Med. 2023 Mar;29(3):632-645. doi: 10.1038/s41591-022-02178-3. Epub 2023 Mar 16. PMID: 36928817; PMCID: PMC10033443.
  • Calsina B, Piñeiro-Yáñez E, Martínez-Montes ÁM, Caleiras E, Fernández-Sanromán Á, Monteagudo M, Torres-Pérez R, Fustero-Torre C, Pulgarín-Alfaro M, Gil E, Letón R, Jiménez S, García-Martín S, Martin MC, Roldán-Romero JM, Lanillos J, Mellid S, Santos M, Díaz-Talavera A, Rubio Á, González P, Hernando B, Bechmann N, Dona M, Calatayud M, Guadalix S, Álvarez-Escolá C, Regojo RM, Aller J, Del Olmo-Garcia MI, López-Fernández A, Fliedner SMJ, Rapizzi E, Fassnacht M, Beuschlein F, Quinkler M, Toledo RA, Mannelli M, Timmers HJ, Eisenhofer G, Rodríguez-Perales S, Domínguez O, Macintyre G, Currás-Freixes M, Rodríguez-Antona C, Cascón A, Leandro-García LJ, Montero-Conde C, Roncador G, García-García JF, Pacak K, Al-Shahrour F, Robledo M. Genomic and immune landscape of metastatic pheochromocytoma and paraganglioma. Nat Commun. 2023 Feb 28;14(1):1122. doi: 10.1038/s41467-023-36769-6. PMID: 36854674; PMCID: PMC9975198.
  • Fernández S, Solórzano JL, Díaz E, Menéndez V, Maestre L, Palacios S, López M, Colmenero A, Estévez M, Montalbán C, Martínez Á, Roncador G, García JF. JAK/STAT blockade reverses the malignant phenotype of Hodgkin and Reed-Sternberg cells. Blood Adv. 2023 Aug 8;7(15):4135-4147. doi: 10.1182/bloodadvances.2021006336. PMID: 36459489; PMCID: PMC10407154.
  • Yamamoto Y, Carreras J, Shimizu T, Kakizaki M, Kikuti YY, Roncador G, Nakamura N, Kotani A. Anti-HBV drug entecavir ameliorates DSS-induced colitis through PD-L1 induction. Pharmacol Res. 2022 May;179:105918. doi: 10.1016/j.phrs.2021.105918. Epub 2022 Jan 11. PMID: 35031477.
  • Miyaoka M, Kikuti YY, Carreras J, Ito A, Ikoma H, Tomita S, Kawada H, Roncador G, Bea S, Campo E, Nakamura N. Copy Number Alteration and Mutational Profile of High-Grade B-Cell Lymphoma with MYC and BCL2 and/or BCL6 Rearrangements, Diffuse Large B-Cell Lymphoma with MYC-Rearrangement, and Diffuse Large B-Cell Lymphoma with MYC-Cluster Amplification. Cancers (Basel). 2022 Nov 27;14(23):5849. doi: 10.3390/cancers14235849. PMID: 36497332; PMCID: PMC9736204.
  • Carreras J, Roncador G, Hamoudi R. Artificial Intelligence Predicted Overall Survival and Classified Mature B-Cell Neoplasms Based on Immuno-Oncology and Immune Checkpoint Panels. Cancers (Basel). 2022 Oct 28;14(21):5318. doi: 10.3390/cancers14215318. PMID: 36358737; PMCID: PMC9657332.
  • Pileri A, Cavicchi M, Bertuzzi C, Righi S, Zengarini C, Sabattini E, Roncador G, Agostinelli C. TOX Expression in Mycosis Fungoides and Sezary Syndrome. Diagnostics (Basel). 2022 Jun 29;12(7):1582. doi: 10.3390/diagnostics12071582. PMID: 35885488; PMCID: PMC9316398.
  • Roncador G, Puñet-Ortiz J, Maestre L, Rodríguez-Lobato LG, Jiménez S, Reyes-García AI, García-González Á, García JF, Piris MÁ, Montes-Moreno S, Rodríguez-Justo M, Mena MP, Fernández de Larrea C, Engel P. CD229 (Ly9) a Novel Biomarker for B-Cell Malignancies and Multiple Myeloma. Cancers (Basel). 2022 Apr 26;14(9):2154. doi: 10.3390/cancers14092154. PMID: 35565280; PMCID: PMC9101303.
  • Righi S, Novero D, Godio L, Bertuzzi C, Bacci F, Agostinelli C, Sagramoso C, Rossi M, Piccioli M, Gazzola A, Mannu C, Roncador G, Sabattini E. Myeloid nuclear differentiation antigen: an aid in differentiating lymphoplasmacytic lymphoma and splenic marginal zone lymphoma in bone marrow biopsies at presentation. Hum Pathol. 2022 Jun;124:67-75. doi: 10.1016/j.humpath.2022.03.008. Epub 2022 Mar 23. PMID: 35339566.
  • Peña-Cardelles JF, Pozo-Kreilinger JJ, Roncador G, Esteban-Hernández J, Moro-Rodríguez JE, Sastre-Perona A, Castelo-Fernández B, Cebrián-Carretero JL. Prognosis Value of Immunoregulatory Molecules in Oral Cancer Microenvironment: An Immunohistochemical Study. Biomedicines. 2022 Mar 19;10(3):710. doi: 10.3390/biomedicines10030710. PMID: 35327512; PMCID: PMC8945047.
  • Carreras J, Hiraiwa S, Kikuti YY, Miyaoka M, Tomita S, Ikoma H, Ito A, Kondo Y, Roncador G, Garcia JF, Ando K, Hamoudi R, Nakamura N. (2021). Artificial Neural Networks Predicted the Overall Survival and Molecular Subtypes of Diffuse Large B-Cell Lymphoma Using a Pancancer Immune-Oncology Panel. Cancers (Basel) 13 (24).
  • Monteagudo M, Martínez P, Leandro-García LJ, Martínez-Montes ÁM, Calsina B, Pulgarín-Alfaro M, Díaz-Talavera A, Mellid S, Letón R, Gil E, Pérez-Martínez M, Megías D, Torres-Ruiz R, Rodriguez-Perales S, González P, Caleiras E, Jiménez-Villa S, Roncador G, Álvarez-Escolá C, Regojo RM, Calatayud M, Guadalix S, Currás-Freixes M, Rapizzi E, Canu L, Nölting S, Remde H, Fassnacht M, Bechmann N, Eisenhofer G, Mannelli M, Beuschlein F, Quinkler M, Rodríguez-Antona C, Cascón A, Blasco MA, Montero-Conde C, Robledo M. Analysis of Telomere Maintenance Related Genes Reveals NOP10 as a New Metastatic-Risk Marker in Pheochromocytoma/Paraganglioma (2021). Cancers (Basel) 13(19): 4758.