EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network homepage



Giovanna Roncador

Name of the laboratory

CNIO: Centro Nacional de Investigaciones Oncológicas
Monoclonal Antibodies Unit
Centro Nacional de Investigaciones Oncológicas
Calle Melchor Fernández Almagro 3
28029 Madrid, Spain
34 912 246 900

Members of the laboratory

Members: Monoclonal Antibodies Unit
Unit Leader
Giovanna Roncador
Lorena Maestre
Ana Isabel Reyes García
Sherezade Jiménez
Álvaro García

Laboratory activity

Hybridoma technology for the production of monoclonal antibodies represents one of the most relevant methodological advances in biomedicine. The availability of monoclonal antibodies has significantly improved our knowledge about cell biology, opening new possibilities for basic and applied research. Its use in medicine has also improved the diagnostics, prevention and treatment of a large number of diseases.

The Monoclonal Antibodies (mAb) Unit provides CNIO Research Groups with the “à la carte”, generation of mAbs which can then be used as tools to characterise new pathways involved in cancer development. We are highly specialised in mouse and rat monoclonal antibodies production. The Unit also offers mAbs production in gene-inactivated mice, mAb characterisation and validation, medium-scale mAb production and a service of Mycoplasma testing for the cell culture facility.

Research activities

Mouse specific antibodies: During the latest years, genetic approaches have been extensively used at the CNIO for the functional analysis of genes directly implicated in the process of tumor transformation and progression. The study of mouse models has been hampered by the absence of reliable mouse-specific antibodies to be used on paraffin-embedded tissues. For this reason, and in collaboration with the CNIO Comparative Pathology Unit, we have tested and developed a panel of high quality mouse specific mAbs, able to detect their target molecules on paraffin mouse tissue sections by immunohistochemistry.

Rat mAb: Many protein targets relevant for signal transduction and disease are highly conserved between mice and humans, and can therefore be recognized as self-antigens by a mouse host. To overcome this problem, this year we have made available in our unit a new service of production of mAbs in rats. This will provide CNIO investigators with a powerful tool to study cell biology and cancer related processes in mouse models. The mAbs against mouse protein and produced in rat to date are directed against the following proteins: Beta-Gal, p15,p16,p21,p53, Hes-1and Nanog.

Techniques available

  • monoclonal antibody production in mice
  • polyclonal antibody production
  • cloning technique
  • antibody characterization
  • development of double and triple immunostaining techniques
  • mycoplasma testing
  • genetic immunization

Publications (2019-present)

  • Calsina B, Piñeiro-Yáñez E, Martínez-Montes ÁM, Caleiras E, Fernández-Sanromán Á, Monteagudo M, Torres-Pérez R, Fustero-Torre C, Pulgarín-Alfaro M, Gil E, Letón R, Jiménez S, García-Martín S, Martin MC, Roldán-Romero JM, Lanillos J, Mellid S, Santos M, Díaz-Talavera A, Rubio Á, González P, Hernando B, Bechmann N, Dona M, Calatayud M, Guadalix S, Álvarez-Escolá C, Regojo RM, Aller J, Del Olmo-Garcia MI, López-Fernández A, Fliedner SMJ, Rapizzi E, Fassnacht M, Beuschlein F, Quinkler M, Toledo RA, Mannelli M, Timmers HJ, Eisenhofer G, Rodríguez-Perales S, Domínguez O, Macintyre G, Currás-Freixes M, Rodríguez-Antona C, Cascón A, Leandro-García LJ, Montero-Conde C, Roncador G, García-García JF, Pacak K, Al-Shahrour F, Robledo M (2023). Genomic and immune landscape Of metastatic pheochromocytoma and paraganglioma. Nat Commun. 28;14(1):1122.
  • Larrayoz M, Garcia-Barchino MJ, Celay J, Etxebeste A, Jimenez M, Perez C, Ordoñez R, Cobaleda C, Botta C, Fresquet V, Roa S, Goicoechea I, Maia C, Lasaga M, Chesi M, Bergsagel PL, Larrayoz MJ, Calasanz MJ, Campos-Sanchez E, Martinez-Cano J, Panizo C, Rodriguez-Otero P, Vicent S, Roncador G, Gonzalez P, Takahashi S, Katz SG, Walensky LD, Ruppert SM, Lasater EA, Amann M, Lozano T, Llopiz D, Sarobe P, Lasarte JJ, Planell N, Gomez-Cabrero D, Kudryashova O, Kurilovich A, Revuelta MV, Cerchietti L, Agirre X, San Miguel J, Paiva B, Prosper F, Martinez-Climent JA (2023). Preclinical models for prediction of immunotherapy outcomes and immune evasion mechanisms in genetically heterogeneous multiple myeloma. Nat Med. 29(3):632-645.
  • Del Rio ML, de Juan CY, Roncador G, Caleiras E, Álvarez-Esteban R, Pérez-Simón JA, Rodriguez-Barbosa (2023). Genetic deletion of HVEM in a leukemia B cell line promotes a preferential increase of PD-1- stem cell-like T cells over PD-1+ T cells curbing tumor progression. Front Immunol. 23;14:1113858.
  • Carreras J, Kikuti YY, Miyaoka M, Hiraiwa S, Tomita S, Ikoma H, Kondo Y, Ito A, Nagase S, Miura H, Roncador G, Colomo L, Hamoudi R, Campo E, Nakamura N.Hematol Rep. Mutational Profile and Pathological Features of a Case of Interleukin-10 and RGS1-Positive Spindle Cell Variant Diffuse Large B-Cell Lymphoma.(2023). Hematol Rep 12;15(1):188-200.
  • Roncador G, Puñet-Ortiz J, Maestre L, Rodríguez-Lobato LG, Jiménez S, Reyes-García AI, García-González Á, García JF, Piris MÁ, Montes-Moreno S, Rodríguez-Justo M, Mena MP, Fernández de Larrea C, Engel P (2022). CD229 (Ly9) a Novel Biomarker for B-Cell Malignancies and Multiple Myeloma. Cancers (Basel) 26 (9).
  • Carreras J, Kikuti YY, Hiraiwa S, Miyaoka M, Tomita S, Ikoma H, Ito A, Kondo Y, Itoh J, Roncador G, Martinez A, Colomo L, Hamoudi R, Ando K, Nakamura N (2022). High PTX3 expression is associated with a poor prognosis in diffuse large B-cell lymphoma. Cancer Sci. 113 (1).
  • Pileri A, Cavicchi M, Bertuzzi C, Righi S, Zengarini C, Sabattini E, Roncador G, Agostinelli C (2022). TOX Expression in Mycosis Fungoides and Sezary Syndrome. Diagnostics (Basel). 29;12(7).
  • Peña-Cardelles JF, Pozo-Kreilinger JJ, Roncador G, Esteban-Hernández J, Moro-Rodríguez JE, Sastre-Perona A, Castelo-Fernández B, Cebrián-Carretero JL (2022). Prognosis Value of Immunoregulatory Molecules in Oral Cancer Microenvironment: An Immunohistochemical Study. Biomedicines 19;10(3).
  • Carreras J, Roncador G, Hamoudi R (2022). Artificial Intelligence Predicted Overall Survival and Classified Mature B-Cell Neoplasms Based on Immuno-Oncology and Immune Checkpoint Panels. Cancers (Basel) 28;14(21).
  • Righi S, Novero D, Godio L, Bertuzzi C, Bacci F, Agostinelli C, Sagramoso C, Rossi M, Piccioli M, Gazzola A, Mannu C, Roncador G, Sabattini E (2022). Myeloid nuclear differentiation antigen: an aid in differentiating lymphoplasmacytic lymphoma and splenic marginal zone lymphoma in bone marrow biopsies at presentation. Hum Pathol 124:67-75.
  • Carreras J, Hiraiwa S, Kikuti YY, Miyaoka M, Tomita S, Ikoma H, Ito A, Kondo Y, Roncador G, Garcia JF, Ando K, Hamoudi R, Nakamura N. (2021). Artificial Neural Networks Predicted the Overall Survival and Molecular Subtypes of Diffuse Large B-Cell Lymphoma Using a Pancancer Immune-Oncology Panel. Cancers (Basel) 13 (24).
  • Monteagudo M, Martínez P, Leandro-García LJ, Martínez-Montes ÁM, Calsina B, Pulgarín-Alfaro M, Díaz-Talavera A, Mellid S, Letón R, Gil E, Pérez-Martínez M, Megías D, Torres-Ruiz R, Rodriguez-Perales S, González P, Caleiras E, Jiménez-Villa S, Roncador G, Álvarez-Escolá C, Regojo RM, Calatayud M, Guadalix S, Currás-Freixes M, Rapizzi E, Canu L, Nölting S, Remde H, Fassnacht M, Bechmann N, Eisenhofer G, Mannelli M, Beuschlein F, Quinkler M, Rodríguez-Antona C, Cascón A, Blasco MA, Montero-Conde C, Robledo M. Analysis of Telomere Maintenance Related Genes Reveals NOP10 as a New Metastatic-Risk Marker in Pheochromocytoma/Paraganglioma (2021). Cancers (Basel) 13(19): 4758.
  • Voskuil JLA, Bandrowski A, Begley CG, Bradbury ARM, Chalmers AD, Gomes AV, Hardcastle T, Lund-Johansen F, Plückthun A, Roncador G, Solache A, Taussig MJ, Trimmer JS, Williams C, Goodman (2020). The Antibody Society's antibody validation webinar series. MAbs 12(1).
  • Maestre L, García-García JF, Jiménez S, Reyes-García AI, García-González Á, Montes-Moreno S, Arribas AJ, González-García P, Caleiras E, Banham AH, Piris MÁ, Roncador G (2020). High-mobility group box (TOX) antibody a useful tool for the identification of B and T cell subpopulations. PLoS One 27, 15(2).