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mAbs Against MOUSE Protein

P16

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:rat anti mouse
CLONE NAME:CAR327C
PROTEIN:cyclin-dependent kinase inhibitor 2A
ANTIGEN USED:HIS-GST-mp16 recombinant protein (full length protein)
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2b
SPECIES REACTIVITY:mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
CAR327C 1:10 supernatant Discovery Xt CC1 OmniMap Ventana Fibrosarcoma tissue / lymph node nuclear
Immunofluorescence
Enlarge image
Anti-mp16 antibody in transfected cells
Hek-Bcl2 trasfected cells was used as negative control.
Enlarge image
CAR327C antibody on mouse tissues.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
CAR327C undiluted supernatant Papilloma extract / 16kDa Available
Immunoprecipitation
Enlarge image
Western blotting of CAR327C antibody
Lane 1 HIS-GST-mp16 recombinant protein (0,1ug) (+)
Lane 2 HEK-TDT transfected cells (20ug) (-)
Lane 3 HEK-V5-p16 transfected cells (20ug) (+)
Lane 4 556P (mouse papiloma extract) (200ug) (+)
Lane 5 556 (normal mouse skin) (200ug) (-)
Lane 6 C108 (MEFs p16/p19 -/-) (200ug) (-)

556P: senescent lesion generated by MBA/TPA in mouse skin