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mAbs Against MOUSE Protein

p53

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:rat anti mouse
CLONE NAME:POE316A
PROTEIN:Mus musculus transformation related protein 53 (Trp53)
ANTIGEN USED:His-GST-mp53 recombinant protein
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

p53 also known as cellular tumor antigen p53 or phosphoprotein p53 or tumor suppressor p53 is a protein that in humans is encoded by the TP53 gene. The p53 protein is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor, preventing cancer. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
POE316A 1:2 supernatant mouse intestine / nuclear
Immunofluorescence
Enlarge image
POE316A mAb in transfected cells
Nuclear and cytoplasmic staining of POE316A mAb in transfected HEK293-mp53-GFP cells. Anti-GFP was used as positive control.
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POE316A mAb in mouse paraffin tissues
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
POE316A neat supernatant supernatant / 43kDa Available
Immunoprecipitation
Enlarge image
Western Blotting of mp53 (POE316A)
Western Blotting of mp53 using different mouse cell lines and HEK293T transfected cells.
Lane 1 Hek-mp53-GFP transfected cells (20ug) (+)
Lane 2 Hek-FGFR1-GFP transfected cells (20ug) (-)
Lane 3 Mefs mp53 ER/ER cells (100ug) (+)
Lane 4 Mefs mp53 ER/ER Ras GFP (100ug) (+)
Enlarge image
Western Blotting of mp53 (POE316A)
Predicted molecular weight: 43kDa
Observed molecular weight: 43kDa

Lanes
Lane 1 KSS mouse 1 lox/lox (30ug) (-)
Lane 2 KSS mouse 4 lox/lox (30ug) (-)
Lane 3 KSS mouse 6 p53 +/+ (30ug) (+)
Lane 4 KSS mouse 7 lox/lox (30ug) (-)
Lane 5 KNR1 mouse+CRE (30ug) (+)
Lane 6 KNR1 mouse+CRE+shp53 (30ug) (+/-)

Image kindly provided by Barbara Oldrini.