mAbs Against MOUSE Protein
TOX
STATUS: | |
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CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
STATUS: | Validated |
TYPE: | Rat anti mouse |
CLONE NAME: | NAN448B |
PROTEIN: | Thymocyte selection-associated high mobility group box protein (TOX) |
PROTEIN WEB: | https://www.uniprot.org/uniprot/O94900 |
ANTIGEN USED: | His-TOX (1-250aa) recombinant protein |
FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
ISOTYPE: | IgG1 |
SPECIES REACTIVITY: | human and mouse |
PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Applications
IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
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Frozen tissue and cytospins | |||||||||
Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | NAN448 | 1:2 | supernatant | pH high | / | nuclear | |||
Immunofluorescence |
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- NAN448 in mouse paraffin lymphoid tissues.
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- TOX expression in rective mouse tissues