mAbs Against MOUSE Protein
TOX
| STATUS: |  |
|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
|---|
| STATUS: | Validated |
|---|
| TYPE: | Rat anti mouse |
|---|
| CLONE NAME: | NAN448B |
|---|
| PROTEIN: | Thymocyte selection-associated high mobility group box protein (TOX) |
|---|
| PROTEIN WEB: | https://www.uniprot.org/uniprot/O94900 |
|---|
| ANTIGEN USED: | His-TOX (1-250aa) recombinant protein |
|---|
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
|---|
| ISOTYPE: | IgG1 |
|---|
| SPECIES REACTIVITY: | human and mouse |
|---|
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
|---|
Applications
IHC Techniques
| IHC Techniques |
Clone |
Dilution |
Antibody concentration |
Antigen retrieval method |
Visualization kit |
+/- control |
Protein localization |
Positivity in other species |
Protocol |
| Frozen tissue and cytospins |
|---|
| Paraffin tissue |
|---|
Recommended Result obtained is satisfactory. The reagent can be use in this application | NAN448 |
1:2 |
supernatant |
pH high |
|
/ |
nuclear |
|
|
| Immunofluorescence |