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mAbs Against HUMAN Protein

CD30

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:CON6D
PROTEIN:Tumor necrosis factor receptor superfamily member 8, Lymphocyte activation antigen CD30
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/P28908.1
ANTIGEN USED:L428 Hodgkin’s lymphoma cell line
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2a
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Pierce Antibodies, Abcam and Santa Cruz

REFERENCES

Lau SK1, Thomas P, Weiss LM. Immunohistochemical evaluation of CON6D/B5: a new CD30 monoclonal antibody. Appl Immunohistochem Mol Morphol. 2010 May;18(3):273-7

Nam-Cha SH, Montes-Moreno S, Salcedo MT, Sanjuan J, Garcia JF and Piris MA. Lymphocyte-rich classical Hodgkin's lymphoma: distinctive tumor and microenvironment markers. Modern Pathology (2009) 22, 1006–1015.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
CON6D Neat supernatant Tonsil /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
CON6D Neat supernatant Citrate Leica Tonsil / Available
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
CON6D Neat supernatant Tonsil /
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CON6D mAb in human frozen tonsil
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CON6D mAb in human paraffin tissues
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CD30 detection in human tonsil by immunofluorescence
CD30 (CON6D) in green, PD1 (NAT105C) in red and DAPI in blue.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
CON6D Neat supernatant OPM2 cell line / 62kDa 62kDa Available
Immunoprecipitation
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CON6D mAb in different human cell lines
Lane 1 DEV cell line (100ug) (+)
Lane 2 KM-H2 cell line (100ug) (-)
Lane 3 Human tonsil (100ug) (-)
Lane 4 LP1 cell line (100ug) (+)
Lane 5 OPM2 cell line (100ug) (+)