mAbs Against HUMAN Protein
CD38
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | MIX187A |
| PROTEIN: | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/P28907#names_and_taxonomy |
| ANTIGEN USED: | LP1 cell line |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Synthesizes the second messengers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | MIX187A | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | MIX187A | Neat | supernatant | Citrate | Novolink | Tonsil / | membrane | Available | |
| Immunofluorescence | |||||||||
Enlarge image
- MIX187A mAb is able to detect human CD38 protein in immunocytochemistry.
- To confirm that MIX187A mAb recognizes human CD38 protein, immunocytochemistry on frozen cytospin preparations of myc-tagged CD38 expressed in HEK293 was performed. Cytospin preparation of PLCG1-myc transfected cells was used as negative control. Anti-MYC antibody was used as positive control.
Enlarge image
- MIX187A antibody can be used to detect CD38 protein in human frozen tissues.
Enlarge image
- MIX187A antibody can be used to detect CD38 protein in human paraffin tissues.
| FLOW CYTOMETRY | Clone | Dilution | +/- control | Type of fluorocrom | Protocol |
|---|---|---|---|---|---|
Recommended Result obtained is satisfactory. The reagent can be use in this application | MIX187A | 1:200 Supernatant | / |
Enlarge image
- MIX187A antibody can be used to detect CD38 in flow cytometry.