mAbs Against HUMAN Protein
|CONTACT INFORMATION:||Immunology Unit, Faculty of Medicine and Medical Sciences, University of Barcelona|
|TYPE:||mouse anti human|
|CLONE NAME:||P.SEL KO 2.3|
|ANTIGEN USED:||300.19 transfected with human CD62P |
|PREPARATION AND STORAGE:||-|
|APP RECOMMENDED:||IP, Flow cytometry|
|APP NO TESTED:||IHQ-paraffin, IHQ-frozen, IF, WB|
P-selectin (CD62P) is an adhesion molecule expressed on the activated endothelium and activated platelets that is involved in the initial
attachment of leukocytes to inflamed vascular endothelium.
P-selectin is constitutively present in alfa-granules of platelets and Weibel-Palade bodies of endothelial cells, and is mobilized to the cell surface after activation by thrombogenic and inflammatory mediators.
Massaguer A, Engel P, Pérez-del-Pulgar S, Bosch J, Pizcueta P. Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P). Tissue Antigens. 2000, 56:117-128
Massaguer A, Perez-Del-Pulgar S, Engel P, Serratosa J, Bosch J, Pizcueta P. Concanavalin-A-induced liver injury is severely impaired in mice deficient in P-selectin. Leucocyte Typing VII.(ed. Mason D et al.), Oxford University Press, Oxford 2002, 342-343
Barrabés JA, Mirabet M, Agulló L, Figueras J, Pizcueta P, Garcia-Dorado D. Platelet deposition in remote cardiac regions after coronary occlusion. Eur J Clin Invest. 2007, 37:939-46
Mirabet M, Garcia-Dorado D, Inserte J, Barrabés JA, Lidón RM, Soriano B, Azevedo M, Padilla F, Agulló L, Ruiz-Meana M, Massaguer A, Pizcueta P, Soler-Soler J. Platelets activated by transient coronary occlusion exacerbate ischemia-reperfusion injury in rat hearts. Am J Physiol Heart Circ Physiol. 2002; 283:H1134-41
Massaguer A, Engel P, Tovar V, March S, Rigol M, Solanes N, Bosch J, Pizcueta P. Characterization of platelet and soluble-porcine P-selectin (CD62P). Vet Immunol Immunopathol. 2003, 96:169-81.
||Positivity in other species
|P.SEL KO 2.3
- Immunoprecipitation of P-selectin from human platelets with P-sel.KO.2.2 and P-sel.KO.2.3 mAbs.
- Human activated and surface biotinylated platelets were lysed and immunoprecipitated with P-sel.KO.2.2 and P-sel.KO.2.3 mAbs. A mAb against CD84 was used as positive control, and an irrelevant mAb as negative control. Samples were run on a SDS-PAGE gel under reducing conditions and blotted onto PVDF membrane.
Membrane was treated with HRP-avidin and the bands developed. Molecular weight markers (kDa) are indicated.
||Type of fluorocrom
|P.SEL KO 2.3
|| / untransfected 300.19 cells
- Specificity of the P-sel.KO.2.3 mAb for P.Selectin by flow cytometry
- Representative histogram obtained after indirect immunofluorescence staining of untransfected 300.19
cells (dashed lines) and 300.19 cells transfected
with human P-selectin c-DNA (solid lines) with P-sel.KO.2.3. Fluorescence intensity is shown on a 4-decade log scale.
||The P-Sel.KO.2.3 mAb, that reacts with human, mouse and rat Pselectin, was used as the capture antibody.