Name of the laboratory
- Immunology Unit
- Department of Cellular Biology and Pathology
- Medical School, University of Barcelona
- 08036 Barcelona, Spain
- 34 932 275 400 (ext.: 4010, 4011)
Members of the laboratory
- Head of the Unit
Pablo Engel Rocamora email@example.com
Xavier Romero Ros firstname.lastname@example.org
- Ph.D. Student
Marta Cuenca Lopera email@example.com
Adriana Lázaro Domínguez firstname.lastname@example.org
The Immunology Unit at the University of Barcelona has focused its the studies on leukocyte cell-surface molecules. Monoclonal antibodies are essential tools for determining the expression and function of these molecules. Monoclonal antibodies produced in our Unit during the last 10 years have been used for research.
Dr. Engel is a member of the International HCDM (Human Cell Differentiation Molecules) Council and has been recently appointment as Chairman of the next Human Leukocyte Differentiation Antigen Workshop and Conference (HLDA9) to be held in Barcelona (2009) (http://www.hcdm.org/).
Characterization of the expression and function of the SLAM (Signaling Lymphocyte-Activation Molecule) family members: Our team has produced several antibodies against the human and mouse cell-surface molecules of the SLAM family including CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). These reagents have been essential in establishing the expression and various functions of this new family of immunoreceptors.
Production and characterization of antibodies against P-selectin and CD69 using KO-mice: We have generated a large panel of monoclonal antibodies against the adhesion molecule P-selectin and CD69. These antibodies have been generated using knock-out KO-mice. A unique characteristic of these antibodies is that they are able to recognize epitopes preserved across multiple species. These antibodies have been used by a large group of collaborators to study the function of P-selectin and CD69 in vitro and in vivo in different animal model of disease.
Other antibodies: Antibodies against other cell-surface molecules recently produced in our lab include TNF receptor 2, human Syndecan-2, mouse GITRL and rat CD38.
Production of antibodies against other cell-surface molecules: We are currently engaged in the production of antibodies that identify new markers for the diagnosis and treatment leukaemia and lymphoma.
- monoclonal antibody production in mice
- monoclonal antibody production in hamsters
- antibody characterization
- multi-colour flow cytometry
- immunization with transfected cells
- Pérez-Carmona N, Farré D, Martínez-Vicente P, Terhorst C, Engel P, Angulo A. SLAM family receptor homologs in New World monkey cytomegaloviruses. J. Virol. 2015 (in press)
- Cuenca M, Romero X, Sintes J, Terhorst C, Engel P. Antibody targeting of Ly9 (CD229) disrupts marginal zone and B1 B cell homeostasis and antibody responses in mice. J. Immunol. 2015 (submitted)
- Yigit B, Halibozeck PJ, Chen S-S, O’Keeffe MS, Arnason J, Avigan D, Gattei V, Chiorazzi N, Wang N, Engel P, Terhorst C. Anti- SLAMF6 in combination with ibrutinib abrogates in vivo expansion of the murine chronic lymphocytic leukemia cells. 2015 (second revision)
- Wang N, Halibozek P, Keszei M, Engel P, Terhorst C Negative regulation of humoral autoimmunity by Slamf6 2015 (submitted)
- van Driel B, Liao G, Engel P, Terhorst C Responses to microbial challenges by SLAMF receptors Front. Immunol. (in press) 2015
- Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS, Šerbec VC, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa JI, Torensma R, Turley H, Pulford K, Banham AH The European Antibody Network’s practical guide to finding and validating suitable antibodies for research mAbs (in press) 2015
- Liao G, van Driel B, Magelky E, O’Keeffe M, de Waal Malefyt R, Engel P, Herzog RW, Mizoguchi E, Bhan AK, Terhorst C Glucocorticoid-induced TNF receptor family related protein ligand regulates the migration of monocytes to the inflamed intestine FASEB J. 2014 28:474-84.
- Zarama A, Pérez-Carmona N, Farré D, Messerle M, Jonjic S, Engel P, Angulo A. Cytomegalovirus m154 Hinders CD48 Cell Surface Expression and Promotes Viral Escape from Host Natural Killer Cell Control PLoS Pathog 2014 10:e1004000. doi: 10.1371/journal.ppat.1004000
- Fenutría F; Martinez VG; Dos Anjos Simões IT; Postigo J; Gil V; Martinez-Florensa M; Sintes J; Naves R; Cashman KS; Alberola-Ila J; Ramos-Casals M; Soldevila G; Raman C; Merino J; Merino R; Engel P; Lozano P. Transgenic expression of soluble human CD5 enhances experimentally-induced autoimmune and anti-tumoral immune responses PLoS ONE 2014 9: e84895. doi:10.1371/journal.pone.0084895
- Romero X, Sintes J, Engel P Role of SLAM family receptors and specific adapter SAP in innate-like lymphocytes Crit Rev Immunol. 2014 34:263-299 De Calisto J, Wang N, Wang G, Yigit B, Engel P, Terhorst C. SAP-independent and –dependent regulation of innate T cell development involving SLAMF receptors Front Immunol. 2014 5:186. (doi: 10.3389/fimmu.2014.00186)
- Romero X, Cañete JD, Engel P. Determination of soluble tumor necrosis factor receptor 2 produced by alternative splicing. Methods Mol Biol. 2014 1155:187-99.
- Sintes J, Cuenca M, Romero X, Bastos R, Terhorst C, Angulo A, Engel P. Ly9 (CD229), a SLAM family receptor, negatively regulates the development of thymic innate memory-like CD8+ T and invariant NKT cells J. Immunol. Cutting edge. 2013 190:21-26.
- Garcia-Recio S, Fuster G, Fernandez-Nogueira P, Pastor-Arroyo EM, Yeon Park S, Mayordomo C, Ametller E, Mancino M, Gonzalez-Farre X, Russnes H, Engel P, Costamagna D, Fernandez PL, Gascon P, Almendro V. Substance P autocrine signaling contributes to persistent HER2 activation that drives malignant progression and drug resistance in breast cancer. Cancer Research 2013 73:6424-34.
- De Salort J, Cuenca M, Terhorst C, Engel P, Romero X. Ly9 (CD229) cell surface receptor is crucial for the development of spontaneous autoantibody production to nuclear antigens. Front Immunol. 2013. 4:225. (doi: 10.3389/fimmu.2013.00225). CLAV
- Liao G, Berger CB, Detre C, Engel P, de Waal Malefyt R, Herzog RW, Bhan AK, Terhorst C Glucocorticoid-Induced Tumor Necrosis Factor Receptor Family-Related Protein Regulates CD4+ T Cell-Mediated Colitis in Mice. Gastroenterology 2012 142:582-591.e8.
- Engel P, Angulo A. Viral immunomodulatory proteins: usurping host genes as a survival strategy. Adv Exp. Med. Biol.. 2012;738:256-76.
- CroatiaStipan JonjicUniversity of Rijeka
- FranceAndré PèlegrinInstitut de Recherche en Cancérologie de Montpellier, IRCM
- FranceArmand BensussanHôpital Saint Louis
- FrancePierre LafayeInstitut Pasteur
- GermanyFriedrich Koch-NolteUniversity Medical Center Hamburg-Eppendorf
- HungaryPeter BaloghClinical Center, University of Pecs
- ItalyFabio MalavasiUniversity of Torino Medical School
- RussiaAlexander FilatovInstitute of Immunology
- RussiaAlexander TaraninInstitute of Molecular and Cellular Biology
- SloveniaVladka Čurin ŠerbecBlood Trasfusion Centre of Slovenia
- SpainGiovanna RoncadorCentro Nacional de Investigaciones Oncológicas
- SpainJosé I. Rodríguez BarbosaInstituto Universitario de Biomedicina
- SpainLeonor KremerCentro Nacional de Biotecnología/CSIC
- SpainNúria PascualNb4D
- SpainPablo EngelMedical School, University of Barcelona
- United KingdomUniversity of OxfordLRF Haemato-oncology Group
- United KingdomVertebrate Antibodies Limited (VAL)
- United KingdomUniversity of OxfordOxFabs
- United KingdomKing's College LondonSt. John's Institute of Dermatology