mAbs Against HUMAN Protein
CLEC9A
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | Rat monoclonal |
| CLONE NAME: | LEIA256A |
| PROTEIN: | C-type lectin domain family 9 member A |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q6UXN8 |
| ANTIGEN USED: | RBL1-CLEC9A-MYC transfected cells |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG2b |
| SPECIES REACTIVITY: | human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | LEIA256A | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | LEIA256A | 1:10 | SUPERNATANT | Spleen / | membrane | Available | |||
| Immunofluorescence | |||||||||
Enlarge image
- LEIA256A mAb is able to detect human CLEC9a protein in immunocytochemistry.
- To confirm that LEIA256B mAb recognizes CLEC9a protein, immunocytochemistry on frozen cytospin preparations of MYC-tagged human CLEC9a expressed in HEK293T was performed. Anti-MYC antibody was used as positive control. Cytospin preparation of MYC-tagged mouse CD68 protein was used as a negative control.
Enlarge image
- LEIA256A mAb can be used to detect CLEC9a protein in human paraffin tissues.