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mAbs Against HUMAN Protein

Estrogen Receptor Alpha

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:CRET94D
PROTEIN:Estrogen Receptor Alpha (ESR1)
PROTEIN WEB:http://www.hprd.org/alternate?hprd_id=00589&isoform_id=00589_4&isoform_name=Isoform_1
ANTIGEN USED:MBP-CRE-ERT recombinant protein
FUSION PARTNER:myeloma p3-NS1/Ag4-1 (NS1) cell
ISOTYPE:IgG1
SPECIES REACTIVITY:Human and mouse
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

DESCRIPTION

The oestrogen receptor alpha (ESR1) is a transcription factor that potentiates the response to diverse stimuli, including oestrogen and growth factors, in various tissue types. Its recruitment to the DNA is directly regulated by the chromatin landscape, inclusive of chromatin compaction and epigenetic modifications.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
CRET94D 1:40 supernatant / Available
Immunofluorescence
Enlarge image
CRET94D in transfected cells
To confirm that CRET94D mAb recognized human Estrogen receptor protein, immunohistochemistry on frozen cytospins preparations of CRE-ERT expressed in Hek293T was performed. Cytospin preparation of E4F1 transfected cells was used as negative control.
Enlarge image
CRET94D in human breast cancer
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
CRET94D 1:5 supernatant / 66kDa mouse Available
Immunoprecipitation
Enlarge image
CRET94D in Western blotting
Lane 1 Hek-CRE-ERT (20ug) (+)
Lane 2 HBL100 cell line (100ug) (-)
Lane 3 MCF7 cell line (100ug) (+)
Lane 4 Human tonsil (100ug) (-)
Lane 5 ZR75-1 cell line (human breast carcinoma) (100ug)(+)