mAbs Against HUMAN Protein
GAPDH
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | FF26A |
| PROTEIN: | Glyceraldehyde-3-phosphate dehydrogenase |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/P04406 |
| ANTIGEN USED: | human CD4+ lymphocytes |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Biolegend and eBioscience |
Description
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules.
References
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Ruehle S, Wager-Miller J, Straiker A, Farnsworth J, Murphy MN, Loch S, Monory K, Mackie K, Lutz B. Discovery and characterization of two novel CB1 receptor splice variants with modified N-termini in mouse. J Neurochem. 2017 Aug;142(4):521-533.
Liu CC, Leclair P, Monajemi M, Sly LM, Reid GS, Lim CJ. α-Integrin expression and function modulates presentation of cell surface calreticulin. Cell Death Dis. 2016 Jun 16;7:e2268.
DiFranco KM, Johnson-Farley N, Bertino JR, Elson D, Vega BA, Belinka BA Jr, Kachlany SC. LFA-1-targeting Leukotoxin (LtxA; Leukothera®) causes lymphoma tumor regression in a humanized mouse model and requires caspase-8 and Fas to kill malignant lymphocytes. Leuk Res. 2015 Jun;39(6):649-56. doi: 10.1016/j.leukres.2015.03.010. Epub 2015 Mar 21.
Butin-Israeli V, Adam SA, Jain N, Otte GL, Neems D, Wiesmüller L, Berger SL, Goldman RD. Role of lamin b1 in chromatin instability. Mol Cell Biol. 2015 Mar;35(5):884-98.
Trifari S, Pipkin ME, Bandukwala HS, Äijö T, Bassein J, Chen R, Martinez GJ, Rao A. MicroRNA-directed program of cytotoxic CD8+ T-cell differentiation. Proceedings of the National Academy of Sciences of the United States of America on 12 November 2013.
Liu CC, Leclair P, Yap SQ, Lim CJ. The Membrane-Proximal KXGFFKR Motif of α-Integrin Mediates Chemoresistance. Molecular and Cellular Biology on 1 November 2013.
Chen CY, Jan YH, Juan YH, Yang CJ, Huang MS, Yu CJ, Yang PC, Hsiao M, Hsu TL, Wong CH. Fucosyltransferase 8 as a functional regulator of nonsmall cell lung cancer. Proceedings of the National Academy of Sciences of the United States of America on 8 January 2013.
Zou L, Cao S, Kang N, Huebert RC, Shah VH. Fibronectin induces endothelial cell migration through β1 integrin and Src-dependent phosphorylation of fibroblast growth factor receptor-1 at tyrosines 653/654 and 766. The Journal of Biological Chemistry on 2 March 2012.
Maestre L, Tooze R, Cañamero M, Montes-Moreno S, Ramos R, Doody G, Boll M, Barrans S, Baena S, Piris MA, Roncador G. XBP1(S) provides a specific marker of stress response and plasma cell differentiation. Haematologica 2009 Mar; 94(3):419-22. Epub 2009 Jan 27.
Aparicio T, Guillou E, Coloma J, Montoya G and Méndez J. The GINS complex interacts with Cdc45 and MCM proteins and is essential for S phase progression in human cells. Nucleic Acids Res. 2009 Apr;37(7):2087-95. Epub 2009 Feb 17.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | FF26A | 1:2 | supernatant | Tonsil / | nuclear | Available | |||
| Immunofluorescence | |||||||||
Enlarge image
- FF26A mAb is able to detect GAPDH protein in human paraffin tissues
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | FF26A | Neat | supernatant | / | 36kDa | 36kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- FF26A mAb is able to detect human and mouse GAPDH protein by WB.
- LANES
Lane 1 U266 cell line (100ug) (+)
Lane 2 Karpas620 cell line (100ug) (+)
Lane 3 LP1 cell line (100ug) (+)
Lane 4 RAJI cell line (100ug) (+)
Lane 5 ALEWIFE cell line (100ug) (+)
Lane 6 SUDHL4 cell line (100ug) (+)