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mAbs Against HUMAN Protein


STATUS:Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
TYPE:mouse monoclonal
PROTEIN:Glyceraldehyde-3-phosphate dehydrogenase
ANTIGEN USED:human CD4+ lymphocytes
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Biolegend and eBioscience


Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules.


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Chen CY, Jan YH, Juan YH, Yang CJ, Huang MS, Yu CJ, Yang PC, Hsiao M, Hsu TL, Wong CH. Fucosyltransferase 8 as a functional regulator of nonsmall cell lung cancer. Proceedings of the National Academy of Sciences of the United States of America on 8 January 2013.

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IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
FF26A 1:2 supernatant Tonsil / nuclear Available
Enlarge image
FF26A mAb is able to detect GAPDH protein in human paraffin tissues
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
FF26A Neat supernatant / 36kDa 36kDa Available
Enlarge image
FF26A mAb is able to detect human and mouse GAPDH protein by WB.

Lane 1 U266 cell line (100ug) (+)
Lane 2 Karpas620 cell line (100ug) (+)
Lane 3 LP1 cell line (100ug) (+)
Lane 4 RAJI cell line (100ug) (+)
Lane 5 ALEWIFE cell line (100ug) (+)
Lane 6 SUDHL4 cell line (100ug) (+)