EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network

☰ Menu

mAbs Against HUMAN Protein

GASDERMIN-1

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:GAS120C
PROTEIN:Gasdermin-A (GSDMA)
PROTEIN WEB:http://www.uniprot.org/uniprot/Q96QA5
ANTIGEN USED:His-Gasdermin1 recombinant C-term protein (208-403aa)
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG2b
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
GAS120C neat supernatant /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
GAS120C 1:5 supernatant Tris-EDTA Human breast cancer /
Immunofluorescence
Enlarge image
GAS120C mAb in transfected cells
To confirm that GAS120C mAb recognized human Gasdemin1 protein, immunohistochemistry on frozen cytospins preparations of Gasdermin1 isoforms 1 and 2 expressed in Hek293T was performed. Anti-HA antibody was used as positive control. Hek-Hes1-HA transfected cells were used as negative control.
Enlarge image
GAS120C antibody can be used to detect human Gasdermin1 protein in paraffin embedded tissue samples.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
GAS120C neat supernatant Colo205 cell line / HeLa cell line 49kDa 49kDa Available
Immunoprecipitation
Enlarge image
GAS120C mAb is able to detect Gasdermin1 protein by WB.
Lane 1 Gasdermin-His recombinant protein (20ug) (+)
Lane 2 MCF7 cell line (100ug) (-)
Lane 3 HBL100 cell line (100ug) (-)
Lane 4 Hela cell line (100ug) (-)
Lane 5 Colo205 cell line (100ug)(+)
Lane 6 BL2 cell line (100ug) (-)
Lane 7 Jurkat cell line (100ug) (-)