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mAbs Against HUMAN Protein

GCET1

Antibody Validated
CONTACT INFORMATION:Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:RAM
PROTEIN:full length human GCET1
PROTEIN WEB:http://www.ncbi.nlm.nih.gov/protein/110225347
ANTIGEN USED:HIS-GCET1
FUSION PARTNER:NS1/Ag4-1 (NS1) cells
ISOTYPE:IgG1
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
APP RECOMMENDED:IHQ-paraffin, IHQ-frozen, IF, WB
APP NO TESTED:IP, Flow cytometry

DESCRIPTION

GCET1 (germinal center B-cell expressed transcript 1) belongs to the SERPIN family of proteins and is located within a cluster of SERPIN genes on chromosome 14q32). This protein may play an important role in GC-B cell physiology. Its expression in lymphomas could be important in differential diagnosis and research.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Montes-Moreno S, Roncador G, Maestre L, Martinez N, Sanchez-Verde L, Camacho F, Cannata J, Martinez-Torrecuadrada JL, Shen Y, Chan WC, Piris MA. Gcet1 (centerin), a highly restricted marker for a subset of Germinal Centre-derived lymphomas. Blood. 2007.http://www.ncbi.nlm.nih.gov/pubmed/17898315

REFERENCES

Montes-Moreno S, Roncador G, Maestre L, Martinez N, Sanchez-Verde L, Camacho F, Cannata J, Martinez-Torrecuadrada JL, Shen Y, Chan WC, Piris MA. Gcet1 (centerin), a highly restricted marker for a subset of Germinal Centre-derived lymphomas. Blood. 2007.

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Not recommended Reagent discarded for this application
RAM /
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
RAM 1:4 supernatant ER2 20 min (Tris-EDTA) Novolink Tonsil / cytoplasmic Available
Immunofluorescence
Enlarge image
Cytospin slides of transfected HEK cells.
The transfected HEK cell line with GCET1 showed specific labelling with RAM antibody. HEK-GCET2 transfected cells were used as negative control.
Enlarge image
Immunostaining of RAM antibody on human paraffin tissues.
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
RAM 1:5 supernatant HT cell line / MHHPREB1 cell lines 46kDa 46kDa Available
Immunoprecipitation
Enlarge image
GCET1 expression by Western blot.
The first two rows demonstate the specificity of RAM antibody on HEK transfected cells.
Lane 1 Hek-GCET1-V5 transfected cells (20ug) (+)
Lane 2 Hek-GCET2-V5 transfected cells (20ug) (-)
Lane 3 MHHPREB1 cell line (100ug) (-)
Lane 4 Karpas 422 cell line (100ug) (+)
Lane 5 DB cell line (100ug) (+)
Lane 6 HT cell line (100ug) (+)
Lane 7 OCILY3 cell line (100ug) (+)
OTHERS
OTHERS Title Description Protocol
Recommended Result obtained is satisfactory. The reagent can be use in this application
Immunofluorescence combined with enzymatic staini
Enlarge image
Doble immunofluorescence combined with enzymatic staining in reactive follicles.
Double immunostaining for KI-67 and GCET1 demonstrated that GCET1 positive cells (red) are double-positive with KI-67 (green), whereas some KI-67 positive cells are negative with GCET1.