mAbs Against HUMAN Protein
|CONTACT INFORMATION:||Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas|
|PROTEIN:||Transcription factor HES-1 - Hairy and Enhancer of split-1|
|ANTIGEN USED:||HIS-mHES1 (aa93-aa282 fragment)|
|FUSION PARTNER:||myeloma p3-NS1/Ag4-1 (NS1) cells|
|SPECIES REACTIVITY:||Mouse and human|
|PREPARATION AND STORAGE:||Aliquot and store at 4C. Do not freeze|
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1 (By similarity). Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.
Maraver A, Fernandez-Marcos PJ, Cash TP, Mendez-Pertuz M, Dueñas M, Maietta P, Martinelli P, Muñoz-Martin M, Martínez-Fernández M, Cañamero M, Roncador G, Martinez-Torrecuadrada JL, Grivas D, de la Pompa JL, Valencia A, Paramio JM, Real FX, Serrano M. NOTCH pathway inactivation promotes bladder cancer progression. J Clin Invest. 2015 Feb;125(2):824-30.
|IHC Techniques||Clone||Dilution||Antibody concentration||Antigen retrieval method||Visualization kit||+/- control||Protein localization||Positivity in other species||Protocol|
|Frozen tissue and cytospins|
Recommended Result obtained is satisfactory. The reagent can be use in this application
|HS395A||1:10||supernatant||20min R2||Bond Max||Tonsil /||nuclear||mouse||Available|
- HS395A mAb can be used to detect HES1 protein in human paraffin tissues
- TISSUE SAMPLE human tonsil, epithelium, and lymphomas (DLBCL, Burkitt and MCL)