mAbs Against HUMAN Protein
Integrin aX
| STATUS: | |
|---|---|
| CONTACT INFORMATION: | University of Oxford marketed by Ximbio.com |
| TYPE: | mouse anti human |
| CLONE NAME: | KB90 |
| PROTEIN: | Integrin aX |
| PROTEIN WEB: | https://www.omim.org/entry/151510 |
| ANTIGEN USED: | Hairy cell leukaemia cells |
| FUSION PARTNER: | P3/NS1/1-Ag4.1 |
| ISOTYPE: | IgG1 kappa |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
| COMMERCIALIZED BY: | Ximbio.com |
| APP RECOMMENDED: | FACS IHC WB |
Description
Integrin aX is found on hairy cell leukaemias and malignant histiocytes and on tissue macrophages, monocytes, activated neutrophils and some T-cell clones. Integrin aX has utility for diagnsis of hariy cell leukaemia
Publication describing antibody characterization/validation
Immunology. 1986 Nov;59(3):427-31.
A monoclonal antibody recognizing the p150/95 leucocyte differentiation antigen.
MacDonald SM, Pulford K, Falini B, Micklem K, Mason DY.
PMID: 2431998
https://www.ncbi.nlm.nih.gov/pubmed/2431998
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
| Paraffin tissue | |||||||||
| KB90 | neat | supernatant | Citrate | Dako Envision | Tonsil / | ||||
| Immunofluorescence | |||||||||
Enlarge image
- KB90 Tonsil
- Tonsil stained with neat supernatant from KB90 hybridoma, detection using Dako Envision kit, 20x power image