EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network

☰ Menu

mAbs Against HUMAN Protein

Integrin b3

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:Y2/51
PROTEIN:Integrin ß3 (CD61; GPIIIa)
PROTEIN WEB:https://www.omim.org/entry/173470
ANTIGEN USED:Phytohaemagglutin-stimulated peripheral blood cells
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:FACS IHC IF WB

DESCRIPTION

CD61 is expressed by megakaryocytes, platelets and osteoclasts. Y2/51 is widely used as a marker of megakaryocytes in bone marrow trephines.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

Histopathology. 1988 Sep;13(3):257-67.
The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens.
Gatter KC1, Cordell JL, Turley H, Heryet A, Kieffer N, Anstee DJ, Mason DY.
PMID: 3192191
https://www.ncbi.nlm.nih.gov/pubmed/3192191

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
Y2/51 neat supernatant Citrate Dako envision Tonsil /
Immunofluorescence
Enlarge image
Y2/51 Tonsil
Tonsil stained with neat supernatant from Y2/51 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
Y2/51 Tonsil
Tonsil stained with neat supernatant from Y2/51 hybridoma, detection using Dako Envision kit, 20x power image