mAbs Against HUMAN Protein
KLHL6
| STATUS: |  |
|---|---|
| CONTACT INFORMATION: | Monoclonal Antibodies Unit, Centro Nacional de Investigaciones Oncológicas |
| STATUS: | Validated |
| TYPE: | mouse monoclonal |
| CLONE NAME: | 92C |
| PROTEIN: | Kelch-like protein 6 |
| PROTEIN WEB: | https://www.uniprot.org/uniprot/Q8WZ60 |
| ANTIGEN USED: | HIS-KLHL6 recombinant protein full length |
| FUSION PARTNER: | NS1/Ag4-1 (NS1) cells |
| ISOTYPE: | IgG1 |
| SPECIES REACTIVITY: | Human |
| PREPARATION AND STORAGE: | Aliquot and store at 4C. Do not freeze |
Description
Involved in B-lymphocyte antigen receptor signaling and germinal center formation.
References
Nam-Cha SH, Montes-Moreno S, Salcedo MT, Sanjuan J, Garcia JF and Piris MA. Lymphocyte-rich classical Hodgkin's lymphoma: distinctive tumor and microenvironment markers. Modern Pathology (2009) 22, 1006–1015.
Applications
| IHC Techniques | Clone | Dilution | Antibody concentration | Antigen retrieval method | Visualization kit | +/- control | Protein localization | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|---|
| Frozen tissue and cytospins | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 92C | Neat | supernatant | / | |||||
| Paraffin tissue | |||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 92C | 1:3 | supernatant | Tris-EDTA | Novolink | Tonsil / Thymus | cytoplasmic | Available | |
| Immunofluorescence | |||||||||
Enlarge image
- 92C mAb is able to detect human KLHL6 protein in immunocytochemistry
- To confirm that 92C mAb recognizes human KLHL6 protein, immunohistochemistry on frozen cytospin preparations of V5-tagged human KLHL6 expressed in HEK293T was performed. Cytospin preparation of TDO2 protein was used as a negative control. Anti-V5 antibody was used to confirm transfection efficiency.
Enlarge image
- 92C mAb can be used to detect KLHL6 protein in human paraffin tissues
| WB Techniques | Clone | Dilution | Antibody concentration | +/- control | Expected MW | Observed Mw | Positivity in other species | Protocol |
|---|---|---|---|---|---|---|---|---|
| Western Blotting | ||||||||
Recommended Result obtained is satisfactory. The reagent can be use in this application | 92C | Neat | supernatant | SUDHL6 cell line / | 70kDa | 70kDa | Available | |
| Immunoprecipitation | ||||||||
Enlarge image
- 92C mAb is able to detect human KLHL6 protein by WB.
- LANES
Lane 1 HEK-KLHL6 (30ug) (+)
Lane 2 HEK-GCET1 (30ug) (-)
Lane 3 SUDHL6 cell line (100ug) (+)
Lane 4 SUDHL4 cell line (100ug) (+)
Lane 5 OCILY3 cell line (100ug) (+)
Lane 6 DEV cell line (100ug) (+)
Lane 7 HT cell line (100ug) (-)
Lane 8 Human tonsil (100ug) (+)
Lane 9 LP1 cell line (100ug) (-)