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mAbs Against HUMAN Protein

LEU2

CONTACT INFORMATION:University of Oxford marketed by Ximbio.com
TYPE:mouse anti human
CLONE NAME:C8/144B
PROTEIN:Leu2 (CD8)
PROTEIN WEB:https://www.omim.org/entry/186910
ANTIGEN USED:Synthetic peptide (the 13 C-terminal residues of the cytoplasmic domain of human CD8a)
FUSION PARTNER:P3/NS1/1-Ag4.1
ISOTYPE:IgG1
SPECIES REACTIVITY:Human
PREPARATION AND STORAGE:Aliquot and store at 4C. Do not freeze
COMMERCIALIZED BY:Ximbio.com
APP RECOMMENDED:IHC IF IP WB

DESCRIPTION

CD8 is a T cell co-receptor that recognises, together with the T cell receptor, MHC class I molecules. CD8 is present on human suppressor/cytotoxic T cells which make up 30% of circulating T cells. C8/144B can be used to detect these cells in tissue sections and also their neoplastic counterparts.

PUBLICATION DESCRIBING ANTIBODY CHARACTERIZATION/VALIDATION

J Clin Pathol. 1992 Dec;45(12):1084-8.
Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence.
Mason DY1, Cordell JL, Gaulard P, Tse AG, Brown MH.
PMID: 1479035
https://www.ncbi.nlm.nih.gov/pubmed/1479035

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Paraffin tissue
C8/144B neat supernatant Tris EDTA Dako Envision Tonsil /
Immunofluorescence
Enlarge image
C8/144B Tonsil
Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 4x power image
Enlarge image
C8/144B Tonsil
Tonsil stained with neat supernatant from bcl-2/100 hybridoma, detection using Dako Envision kit, 20x power image