EuroMAbNet: European Monoclonal Antibodies NetworkEuroMAbNet. European Monoclonal Antibodies Network

☰ Menu

mAbs Against HUMAN Protein

Leucocyte specific protein 1

Antibody Validated
CONTACT INFORMATION:LRF Haemato-oncology Group, University of Oxford
STATUS:Validated
TYPE:mouse anti human
CLONE NAME:TPD153
PROTEIN:LSP-1
ANTIGEN USED:Tonsil cells
FUSION PARTNER:NS1
ISOTYPE:IgG1
SPECIES REACTIVITY:human
PREPARATION AND STORAGE:Aliquot and store at 4ºC. Do not freeze
APP RECOMMENDED:IHQ-frozen, IHQ-paraffin, IF, WB, IP, Flow cytometry

DESCRIPTION

The antibody detects an intracellular antigen in peripheral blood B cells, monocytes and granulocytes and the 85-90% circulating T cells. Medullary but not cortical thymocytes express LSP1. Plasma cells, dendritic cells and Langerhans cells were also LSP1+ve. The protein was detected in a wide range of leukaemias and non-Hodgkin s lymphomas and the tumour cells in classical Hodgkin \'s lymphoma. Reciprocal expression was noted between LSP1 and ALK expression in ALCL. A combination of LSP1 and anti-CD45 may be useful for identifying haematologucal malignancies which have decreased CD45 expression. LSP1 expression is also of use for the diagnosis of T-cell rich B-cell lymphoma from lymphocyte-rich Hodgkin s lymphoma.

REFERENCES

Marafioti T, Mancini C, Ascani S, Sabattini E, Zinzani PL, Pozzobon M, Pulford K, Falini B, Jaffe ES, Müller-Hermelink HK, Mason DY, Pileri SA. Leukocyte-specific phosphoprotein-1 and PU.1: two useful markers for distinguishing T-cell-rich B-cell lymphoma from lymphocyte-predominant Hodgkin \'s disease.
Haematologica. 2004 Aug;89(8):957-64.

Marafioti T, Jabri L, Pulford K, Brousset P, Mason DY, Delsol G
Leucocyte-specific protein (LSP1) in malignant lymphoma and Hodgkin s disease
Br J Haematol. 2003 Feb;120(4):671-8

Pulford K, Jones M, Banham AH, Haralambieva E, Mason DY
Lymphocyte-specific protein 1: a specific marker of human leucocytes.
Immunology. 1999 Feb;96(2):262-71

APPLICATIONS

IHC Techniques
IHC Techniques Clone Dilution Antibody concentration Antigen retrieval method Visualization kit +/- control Protein localization Positivity in other species Protocol
Frozen tissue and cytospins
Recommended Result obtained is satisfactory. The reagent can be use in this application
TPD153 Neat Supernantant Indirect immunoperoxidase or APAAP anti-BCL2 / Mouse IgG1 Intracellular Not known
Paraffin tissue
Recommended Result obtained is satisfactory. The reagent can be use in this application
TPD153 1/5 Supernantant Envision As above / Universal negative Intracellular
Immunofluorescence
Recommended Result obtained is satisfactory. The reagent can be use in this application
TPD153 1/10 Supernantant Directly conjugated antibody As above / As above Intracellular
Enlarge image
Antibody TPD153 in tansfectants
Antibody TPD153 stained LSP1 transfectants. Inset is vector only control
Enlarge image
Immunofluorescent labelling of thymus to show LSP1 expression.
LSP1 (green) is expressed in the CD3-positive (red) cells preset in the medulla while only a minority of cortical CD3-positive cells express LSP1 (arrow).
WB Techniques
WB Techniques Clone Dilution Antibody concentration +/- control Expected MW Observed Mw Positivity in other species Protocol
Western Blotting
Recommended Result obtained is satisfactory. The reagent can be use in this application
TPD153 Neat Supernatant anti-BCL2 / Mouse IgG1 60 kD 60 kD
Immunoprecipitation
Recommended Result obtained is satisfactory. The reagent can be use in this application
TPD153 As above As above As above / As above 60 kD 60 kD
Enlarge image
Western blotting studies on LSP1
a) Antibody TPD153 recognises LSP1 transfectants (pAB280) but not the empty vector (pBK-CMV).
b) Presence of the 60 kD LSP1 protein in tonsil and a range of haematologically-derived cell lines.
FLOW CYTOMETRY
FLOW CYTOMETRY Clone Dilution +/- control Type of fluorocrom Protocol
Recommended Result obtained is satisfactory. The reagent can be use in this application
TPD513 1/10 Anti-BCL2 / Mouse IgG1 FITC
Enlarge image
Flow cytometry of the LSP1 protein on tonsil and blood cells.
a) LSP1 protein is undetectable in unpermeabilised tonsil cells but expressed in b) more than 95% of permeabilised tonsil cells. c), d), e) and f) show the presence of LSP1 protein in both single and double labelling of permeabilsed peripheral blood cells.